膜锚定 DNA 组装用于能量和电子传递。

Membrane-anchored DNA assembly for energy and electron transfer.

机构信息

Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.

出版信息

J Am Chem Soc. 2009 Mar 4;131(8):2831-9. doi: 10.1021/ja8038294.

PMID:19199439

Abstract

In this work we examine the trapping and conversion of visible light energy into chemical energy using a supramolecular assembly. The assembly consists of a light-absorbing antenna and a porphyrin redox center, which are covalently attached to two complementary 14-mer DNA strands, hybridized to form a double helix and anchored to a lipid membrane. The excitation energy is finally trapped in the lipid phase of the membrane as a benzoquinone radical anion that could potentially be used in subsequent chemical reactions. In addition, in this model complex, the hydrophobic porphyrin moiety acts as an anchor into the liposome positioning the DNA construct on the lipid membrane surface. The results show the suitability of our system as a prototype for DNA-based light-harvesting devices, in which energy transfer from the aqueous phase to the interior of the lipid membrane is followed by charge separation.

摘要

在这项工作中，我们使用超分子组装研究了可见光能量的捕获和转化为化学能。该组装由一个吸光天线和一个卟啉氧化还原中心组成，它们通过共价键连接到两条互补的 14 聚体 DNA 链上，杂交形成双螺旋，并锚定在脂质膜上。激发能量最终作为苯醌自由基阴离子被捕获在膜的脂质相中，该自由基阴离子有可能在随后的化学反应中被利用。此外，在这个模型配合物中，疏水性卟啉部分作为一个锚定点将 DNA 构建体定位在脂质膜表面上。结果表明，我们的系统适合作为基于 DNA 的光收集器件的原型，其中能量从水相向脂质膜内部的转移伴随着电荷分离。