通过应用离子依赖性脱氧核酶的超分子构建体对UO22+进行传感及逻辑门设计。

Sensing of UO22+ and design of logic gates by the application of supramolecular constructs of ion-dependent DNAzymes.

作者信息

Moshe Michal, Elbaz Johann, Willner Itamar

机构信息

Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

出版信息

Nano Lett. 2009 Mar;9(3):1196-200. doi: 10.1021/nl803887y.

DOI:10.1021/nl803887y

PMID:19199475

Abstract

Supramolecular constructs composed of ion-dependent DNAzymes and their substrates were used to develop DNAzyme cascades that enabled the sensitive detection of UO22+ or the activation of logic gate operations. The supramolecular complex between the UO22+-dependent DNAzyme and its substrate leads, in the presence of UO22+, to the cleavage of the substrate and to the release of the HRP-mimicking DNAzyme that enables the optical analysis of UO22+ (detection limit 1 x 10-9 M). Similarly, supramolecular complexes between the Mg2+- and UO22+-dependent DNAzymes and tailored substrates enables the design of the "OR" and "AND" logic gates, using Mg2+ and UO22+ as inputs.

摘要

由离子依赖性脱氧核酶及其底物组成的超分子构建体被用于开发脱氧核酶级联反应，该反应能够灵敏检测UO22+或激活逻辑门操作。在UO22+存在的情况下，UO22+依赖性脱氧核酶与其底物之间的超分子复合物会导致底物的切割，并释放出模拟辣根过氧化物酶（HRP）的脱氧核酶，从而实现对UO22+的光学分析（检测限为1×10-9 M）。同样，Mg2+和UO22+依赖性脱氧核酶与定制底物之间的超分子复合物能够以Mg2+和UO22+作为输入，设计出“或”和“与”逻辑门。