North Carolina State University, 2147 Burlington Laboratories, Raleigh, North Carolina 27695, USA.
Anal Chem. 2009 Mar 1;81(5):2013-6. doi: 10.1021/ac802247y.
This report highlights methodologies that enable statistically significant data to be collected for single nucleotide polymorphisms using surface enhanced Raman spectroscopy. Single-stranded oligonucleotides functionalized with 40 nm gold nanoparticles are hybridized with oligonucleotides adsorbed to a photolithographically defined gold surface thus creating a surface enhanced Raman environment around the DNA duplex. With this design characteristic Raman spectra have been collected and explored for differences between DNA duplexes formed from complementary oligonucleotides, completely mismatched oligonucleotides, and those formed from oligonucleotides that have a midsequence single nucleotide mismatch. The results show that statistically significant differences in Raman intensity for characteristic peaks can be collected for the three cases.
本报告重点介绍了使用表面增强拉曼光谱法对单核苷酸多态性进行统计学上有意义的数据收集的方法。用 40nm 金纳米粒子功能化的单链寡核苷酸与吸附在光刻定义的金表面上的寡核苷酸杂交，从而在 DNA 双链体周围创建表面增强拉曼环境。通过这种设计特点，已经收集并探索了拉曼光谱，以研究互补寡核苷酸、完全错配寡核苷酸和具有中序列单个核苷酸错配的寡核苷酸形成的 DNA 双链体之间的差异。结果表明，对于三种情况，都可以收集到特征峰的拉曼强度有统计学意义的差异。
