使用电喷雾电离质谱法研究非共价相互作用。

Using electrospray ionisation mass spectrometry to study non-covalent interactions.

作者信息

McCullough Bryan J, Gaskell Simon J

机构信息

The Michael Barber Centre for Mass Spectrometry, The University of Manchester, Manchester Interdisciplinary Biocentre, The John Garside Building, 131 Princess Street, Manchester M17DN, UK.

出版信息

Comb Chem High Throughput Screen. 2009 Feb;12(2):203-11. doi: 10.2174/138620709787315463.

DOI:10.2174/138620709787315463

PMID:19199888

Abstract

The key strengths of electrospray over any other ionisation techniques are its soft nature and its ability to produce multiply charged ions. This combination is ideal for the study of non-covalent interactions. In this review article, we cover the basics of studying non-covalent interactions by mass spectrometry--illustrated with examples from our own and other labs--and discuss the current mass spectrometry based methods used for understanding and characterising non-covalent protein complexes.

摘要

与其他任何电离技术相比，电喷雾的关键优势在于其温和的特性以及产生多电荷离子的能力。这种组合对于研究非共价相互作用而言非常理想。在这篇综述文章中，我们阐述了通过质谱研究非共价相互作用的基础知识——以我们自己实验室和其他实验室的实例加以说明——并讨论了当前用于理解和表征非共价蛋白质复合物的基于质谱的方法。