Chingin Konstantin, Barylyuk Konstantin, Chen Huanwen
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Guanglan Road 418, Nanchang, Jiangxi 330013, People's Republic of China
Department of Biochemistry, University of Cambridge, Hopkins Building, Tennis Court Road, Cambridge CB2 1QW, UK.
Philos Trans A Math Phys Eng Sci. 2016 Oct 28;374(2079). doi: 10.1098/rsta.2015.0377.
The application range of electrospray ionization mass spectrometry for the quantitative determination of stoichiometries and binding constants for non-covalent protein complexes is broadly discussed. The underlying fundamental question is whether or not the original molecular equilibrium can be preserved during the ionization process and be revealed by subsequent mass spectrometry analysis. Here, we take a new look at this question by discussing recent studies in droplet chemistry.This article is part of the themed issue 'Quantitative mass spectrometry'.
本文广泛讨论了电喷雾电离质谱在定量测定非共价蛋白质复合物的化学计量和结合常数方面的应用范围。其潜在的基本问题是，原始分子平衡在电离过程中能否得以保持，并通过后续的质谱分析揭示出来。在此，我们通过讨论液滴化学方面的最新研究，重新审视这一问题。本文是“定量质谱”主题特刊的一部分。
