Jin R Y, Guo Y R, Wang C M, Wu J X, Zhu G N
Inst. of Pesticide and Environmental Toxicology, Zhejiang Univ., Hangzhou, China.
J Food Sci. 2009 Jan-Feb;74(1):T1-6. doi: 10.1111/j.1750-3841.2008.01002.x.
A mouse hybrid hybridoma (tetradoma) was derived from fusing hybridomas producing monoclonal antibody to N-methylcarbamate pesticide carbofuran with hybridomas producing monoclonal antibody to organophosphorus pesticide Triazophos. The prepared tetradoma line (12C1 to 2H12) secreted hybrid immunoglobulin exhibiting parental and bispecific binding characteristics. The effect of relevant physicochemical factors on the immunoassay based on the 12C1 to 2H12 bispecific monoclonal antibody had been studied to optimize the ELISA performance. The developed immunoassay showed that the detection limit (I(20)) were 0.36 and 1.89 ng/mL for triazophos and carbofuran, respectively, without obvious cross-reactivity to other related compounds. Water samples spiked with triazophos at 0.5, 1, and 5 ng/mL or carbofuran at 5, 10, and 20 ng/mL were directly analyzed by the developed ELISA format. The mean recovery of triazophos and carbofuran were 108.1% and 107.5%, with variation coefficient of 15.9% and 17.7%, respectively.
一种小鼠杂交杂交瘤（四元瘤）是通过将产生针对氨基甲酸酯类农药克百威的单克隆抗体的杂交瘤与产生针对有机磷农药三唑磷的单克隆抗体的杂交瘤融合而获得的。制备的四元瘤细胞系（12C1至2H12）分泌的杂交免疫球蛋白表现出亲本和双特异性结合特性。研究了相关物理化学因素对基于12C1至2H12双特异性单克隆抗体的免疫测定的影响，以优化酶联免疫吸附测定（ELISA）性能。所开发的免疫测定表明，三唑磷和克百威的检测限（I(20)）分别为0.36和1.89 ng/mL，对其他相关化合物无明显交叉反应。分别向水样中添加浓度为0.5、1和5 ng/mL的三唑磷或5、10和20 ng/mL的克百威，然后通过所开发的ELISA方法直接进行分析。三唑磷和克百威的平均回收率分别为108.1%和107.5%，变异系数分别为15.9%和17.7%。
