Wang Kening, Mahalingam Gowtham, Imai Yumi, Pesnicak Lesley, Margolis Todd P, Straus Stephen E, Cohen Jeffrey I
Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, 10 Center Drive, Room 11N234 Bethesda, MD 20892, USA.
Virology. 2009 Mar 30;386(1):79-87. doi: 10.1016/j.virol.2008.12.035. Epub 2009 Feb 6.
We performed in situ hybridization to determine the cell type specific accumulation of the intron of the latency-associated transcript (LAT) in tissues in HSV-2 LAT transgenic mice in which LAT expression is driven by its native promoter. We identified LAT in multiple cell types in most tissues analyzed from HSV-2 LAT transgenic mice. While weak to moderate signals were seen in brain and spinal cord neurons, epithelial cells, and muscle cells, the strongest signals were detected in neurons from dorsal root and trigeminal ganglia. About 70-86% of neurons in these ganglia were LAT-positive with varying signal intensities, while cells surrounding the neurons were LAT-negative. The frequency of A5 or KH10-positive neurons was similar in LAT-positive and total neurons. These data indicate that HSV-2 LAT promoter activity is not restricted to neurons and that LAT accumulation in ganglionic neurons is likely regulated by cell-specific factors.
我们进行了原位杂交，以确定在由其天然启动子驱动LAT表达的HSV-2 LAT转基因小鼠的组织中，潜伏相关转录本（LAT）内含子在细胞类型特异性的积累情况。我们在分析的来自HSV-2 LAT转基因小鼠的大多数组织中的多种细胞类型中鉴定出了LAT。虽然在脑和脊髓神经元、上皮细胞和肌肉细胞中观察到弱至中等信号，但在背根神经节和三叉神经节的神经元中检测到最强信号。这些神经节中约70-86%的神经元LAT呈阳性，信号强度各不相同，而神经元周围的细胞LAT呈阴性。LAT阳性神经元和总神经元中A5或KH10阳性神经元的频率相似。这些数据表明，HSV-2 LAT启动子活性并不局限于神经元，神经节神经元中LAT的积累可能受细胞特异性因子调控。
