展示富含精氨酸肽的生物纳米胶囊的构建。

Construction of arginine-rich peptide displaying bionanocapsules.

作者信息

Shishido Takuya, Yonezawa Daisaku, Iwata Kiyokazu, Tanaka Tsutomu, Ogino Chiaki, Fukuda Hideki, Kondo Akihiko

机构信息

Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

出版信息

Bioorg Med Chem Lett. 2009 Mar 1;19(5):1473-6. doi: 10.1016/j.bmcl.2009.01.049. Epub 2009 Jan 22.

DOI:10.1016/j.bmcl.2009.01.049

PMID:19200722

Abstract

Bionanocapsule (BNC) is a hollow nanoparticle composed of L-protein of the hepatitis B virus surface antigen. BNC can deliver genes or drugs into specific human hepatocytes, but delivery is limited to hepatocytes. In this study, we attempted to alter the specificity of BNCs by genetically introducing cell-penetrating peptides (CPPs), such as arginine-rich peptides, into BNCs. The CPP-fused BNC was efficiently internalized into various cell lines in a short period without significant cytotoxicity. These results show that CPP-BNC could be applied as an efficient carrier for gene and drug delivery.

摘要

生物纳米胶囊（BNC）是一种由乙型肝炎病毒表面抗原的L蛋白组成的空心纳米颗粒。BNC可以将基因或药物递送至特定的人类肝细胞，但递送仅限于肝细胞。在本研究中，我们试图通过基因方式将细胞穿透肽（CPP），如富含精氨酸的肽，引入BNC来改变其特异性。融合了CPP的BNC在短时间内可有效内化进入各种细胞系，且无明显细胞毒性。这些结果表明，CPP-BNC可作为基因和药物递送的有效载体。