Ensenat-Waser Roberto, Pellicer Antonio, Simon Carlos
Spanish Stem Cell Bank, Prince Felipe Research Centre (CIFP), Valencia, Spain.
Fertil Steril. 2009 Apr;91(4):971-4. doi: 10.1016/j.fertnstert.2008.12.034. Epub 2009 Feb 6.
Human embryonic stem cells have the greatest potential for regenerative and reproductive medicine; unfortunately, their full application is limited by ethical and technical problems. In order to overcome these problems and get patient specific pluripotent cells from somatic cells, the field of reprogramming has moved to the generation of induced pluripotent stem cells by overexpression of four transcription factors after viral infection of the patient's somatic cells. In the present paper we review the limitations and milestones of this technique in both regenerative and reproductive medicine.
人类胚胎干细胞在再生医学和生殖医学方面具有最大的潜力；不幸的是，它们的全面应用受到伦理和技术问题的限制。为了克服这些问题并从体细胞中获得患者特异性多能干细胞，重编程领域已转向通过在患者体细胞进行病毒感染后过表达四种转录因子来生成诱导多能干细胞。在本文中，我们综述了该技术在再生医学和生殖医学中的局限性和里程碑。
