González Jaime Fernando, Reimschuessel Renate, Shaikh Badar, Kane Andrew S
School of Veterinary Medicine and Animal Science, Universidad Nacional de Colombia, AA 146224 Bogotá, Colombia.
Mar Environ Res. 2009 May-Jun;67(4-5):183-8. doi: 10.1016/j.marenvres.2009.01.002. Epub 2009 Jan 18.
Hepatic microsomes and cytosols of channel catfish (Ictalurus punctatus), rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar), red tilapia (Oreochromis sp.), largemouth bass (Micropterussalmoides), striped bass (Morone saxatilis), hybrid striped bass (M. saxatilis x M. crysops), and bluegill (Lepomis macrochuris) (n=8) were used to study the kinetics of phase I (ECOD, EROD, PROD, BROD) and phase II (UDP-glucuronosyltransferase (UDPGT)-, sulfotransferase (ST)- and glutathione-s-transferase (GST)-mediated) reactions. The best catalytic efficiency for ECOD and GST activities was performed by channel catfish, Atlantic salmon, rainbow trout and tilapia. The highest EROD catalytic efficiency was for Atlantic salmon. None of the species had either PROD or BROD activities. Rainbow trout had very similar UDPGT catalytic efficiency to tilapia, channel catfish, Atlantic salmon, largemouth bass and bluegill. Sulfotransferase conjugation had no significant differences among the species. In summary, tilapia, channel catfish, Atlantic salmon and rainbow trout had the best biotransforming capabilities; striped bass, hybrid striped bass and bluegill were low metabolizers and largemouth bass shared some capabilities with both groups.
利用斑点叉尾鮰（Ictalurus punctatus）、虹鳟（Oncorhynchus mykiss）、大西洋鲑（Salmo salar）、红罗非鱼（Oreochromis sp.）、大口黑鲈（Micropterus salmoides）、条纹鲈（Morone saxatilis）、杂交条纹鲈（M. saxatilis×M. crysops）和蓝鳃太阳鱼（Lepomis macrochuris）（n = 8）的肝微粒体和胞质溶胶，研究I相（乙氧基异吩恶唑酮脱乙基酶（ECOD）、7-乙氧基香豆素-O-脱乙基酶（EROD）、苯并芘羟化酶（PROD）、联苯双酯羟化酶（BROD））和II相（尿苷二磷酸葡萄糖醛酸基转移酶（UDPGT）、磺基转移酶（ST）和谷胱甘肽-S-转移酶（GST）介导）反应的动力学。斑点叉尾鮰、大西洋鲑、虹鳟和罗非鱼对ECOD和GST活性的催化效率最佳。大西洋鲑对EROD的催化效率最高。所有物种均无PROD或BROD活性。虹鳟的UDPGT催化效率与罗非鱼、斑点叉尾鮰、大西洋鲑、大口黑鲈和蓝鳃太阳鱼非常相似。磺基转移酶结合在各物种之间无显著差异。总之，罗非鱼、斑点叉尾鮰、大西洋鲑和虹鳟具有最佳的生物转化能力；条纹鲈、杂交条纹鲈和蓝鳃太阳鱼是低代谢者，大口黑鲈在两组中均具有一些代谢能力。
