Didier P J, Didier E S, Orenstein J M, Shadduck J A
Department of Pathology, Tulane Regional Primate Research Center, Tulane University, Covington, Louisiana 70433.
J Protozool. 1991 Sep-Oct;38(5):502-7. doi: 10.1111/j.1550-7408.1991.tb04824.x.
Encephalitozoon hellem is a new human microsporidian isolated from corneal biopsies and conjunctival scrapings of three AIDS patients and cultured in Madin Darby canine kidney (MDCK) cells. Encephalitozoon hellem and Encephalitozoon cuniculi display different protein profiles with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and unique antibody binding patterns with murine antisera against Western blots of each organism. Developmental stages of E. hellem in culture are similar to E. cuniculi. Meronts are 1.3-2.7 microns in diameter, develop within a parasitophorous vacuole adjacent to the vacuolar membrane, divide by binary fission, and contain one or two discrete nuclei. Sporonts measure 2 x 3 microns, separate from the vacuolar membrane, and have a thickened outer membrane. Sporoblasts display a tri-layered wall and possess the earliest recognized polar filaments. Mature spores measure 1 x 1.5 microns and are more electron-dense than other stages. Each spore contains a single nucleus, a polar tubule with four to nine coils, thin electron-dense exospore and thick, electron-lucent endospore. Although E. hellem and E. cuniculi differ biochemically and immunologically, their fine structure and development are indistinguishable.
海伦脑炎微孢子虫是从三名艾滋病患者的角膜活检组织和结膜刮片中分离出的一种新的人类微孢子虫，并在麦迪逊-达比犬肾（MDCK）细胞中培养。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析时，海伦脑炎微孢子虫和兔脑炎微孢子虫显示出不同的蛋白质谱，并且与针对每种生物体的蛋白质印迹的鼠抗血清具有独特的抗体结合模式。海伦脑炎微孢子虫在培养中的发育阶段与兔脑炎微孢子虫相似。裂殖体直径为1.3-2.7微米，在靠近液泡膜的寄生泡内发育，通过二分裂进行分裂，并含有一个或两个离散的细胞核。孢子体大小为2×3微米，与液泡膜分离，外膜增厚。孢子母细胞具有三层壁，并拥有最早被识别的极丝。成熟孢子大小为1×1.5微米，比其他阶段的孢子电子密度更高。每个孢子含有一个单核、一个具有四至九个盘绕的极管、薄的电子致密外孢子和厚的、电子透明的内孢子。尽管海伦脑炎微孢子虫和兔脑炎微孢子虫在生化和免疫方面存在差异，但它们的精细结构和发育情况难以区分。
