Zeng Jian Qiang, Deng Zhi Hui, Yang Bao Cheng, Liang Yan Lian, Su Yu Qing, Li Da Cheng, Yu Qiong
Shen Zhen Blood Center, Shen Zhen Institute of Transfusion Medicine, Shen Zhen, Guangdong, China.
Ann Clin Lab Sci. 2009 Winter;39(1):38-42.
The Lutheran blood group is a complex system consisting of 19 identified antigens. It belongs to the immunoglobulin family of receptors and adhesion molecules. Four pairs of antigens show allelic relationships while other antigens are of very high incidence. In this study, we performed genetic polymorphism analyses by molecular techniques of the Lutheran blood group system in Chinese subjects. Blood samples were collected from randomly-selected healthy donors and analyzed by PCR-RFLP or SBT methods. LU1/2(Lu(a)/Lu(b)), LU6/9, LU8/14, and LU18/LU19(Au(a)/Au(b)) antigen polymorphisms were detected as follows: 1102 individuals were diagnosed as Lu(a-b+) type; 117 individuals were all LU(6+9-) genotypes; 119 individuals were all LU(8+14-) genotypes. Among 368 individuals, 278 showed homozygous nt1615A, 6 showed homozygous nt1615G, and 84 showed nt1615A/G heterozygosity. The gene frequencies of Au(a) and Au(b) in Chinese subjects were 0.8695 and 0.1304 respectively. A novel allele was identified in 4 Lu(18+19-) phenotype cases from 3 families.
路德血型系统是一个复杂的系统，由19种已确定的抗原组成。它属于受体和黏附分子的免疫球蛋白家族。四对抗原表现出等位基因关系，而其他抗原的发生率很高。在本研究中，我们采用分子技术对中国人群的路德血型系统进行了基因多态性分析。从随机选择的健康献血者中采集血样，并通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）或测序分型（SBT）方法进行分析。检测到LU1/2（Lu(a)/Lu(b)）、LU6/9、LU8/14和LU18/LU19（Au(a)/Au(b)）抗原多态性如下：1102人被诊断为Lu(a-b+)型；117人全部为LU(6+9-)基因型；119人全部为LU(8+14-)基因型。在368人中，278人显示纯合子nt1615A，6人显示纯合子nt1615G，84人显示nt1615A/G杂合性。中国人群中Au(a)和Au(b)的基因频率分别为0.8695和0.1304。在来自3个家族的4例Lu(18+19-)表型病例中鉴定出一个新的等位基因。
