Hu Zhi Gang, Wang Han Dong, Jin Wei, Yin Hong Xia
Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu, China.
Ann Clin Lab Sci. 2009 Winter;39(1):76-83.
Ketone bodies have been shown to be favorable alternative metabolic substrates and are protective under neuropathologies. At the same time, cytochrome c release has been reported following traumatic brain injury (TBI) and precipitates apoptosis via the mitochondrial pathway. The present study investigated the effects of a ketogenic diet (KD) on TBI. TBI was produced using the Feeney weight-drop model and the animals were fed either normal diet (ND) or KD. Brain edema was estimated by wet/dry weight ratio; cytochrome c was detected by Western blotting; cellular apoptosis in the penumbra area was examined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and active caspase-3 immunohistochemical staining. The results show that brain edema, cytochrome c release, and cellular apoptosis were induced after TBI and that KD reduced these changes dramatically. These findings suggest that KD has potential therapeutic benefit in TBI.
酮体已被证明是有益的替代代谢底物，并且在神经病理学情况下具有保护作用。同时，有报道称创伤性脑损伤（TBI）后会出现细胞色素c释放，并通过线粒体途径引发细胞凋亡。本研究调查了生酮饮食（KD）对TBI的影响。采用Feeney重物坠落模型制造TBI，动物分别喂食正常饮食（ND）或KD。通过湿/干重比评估脑水肿；通过蛋白质免疫印迹法检测细胞色素c；通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）和活性半胱天冬酶-3免疫组织化学染色检查半暗带区域的细胞凋亡。结果表明，TBI后会诱导脑水肿、细胞色素c释放和细胞凋亡，而KD可显著减轻这些变化。这些发现表明，KD对TBI具有潜在的治疗益处。
