Yamanaka Takuya, Miyama Masashi, Tada Yuichi
Katayanagi Institute, Tokyo University of Technology, Hachioji, Japan.
Biosci Biotechnol Biochem. 2009 Feb;73(2):304-10. doi: 10.1271/bbb.80513. Epub 2009 Feb 7.
To identify key genes in the regulation of salt tolerance in the mangrove plant Bruguiera gymnorhiza, transcriptome profiling in the lateral and main roots under conditions of salt stress was performed. Statistical analysis revealed that 175 and 403 of 11,997 genes shoewd significantly increased high expression in the lateral and main roots respectively. One hundred and sixty genes were up-regulated in both types of roots in the early time period, 1 to 12 h after salt treatment. Expression vectors for 28 selected salt responsive genes were constructed and transformed in Agrobacterium tumefaciens, and then screened for salt tolerance. A. tumefaciens transformed with genes for lipid transfer, zinc finger, and ankyrin repeat proteins showed enhanced salt tolerance. Transgenic Arabidopsis plants expressing these three genes also exhibited increased tolerance to NaCl. These results indicate that Agrobacterium functional screening is an effective supplemental method of pre-screening genes involved in abiotic stress tolerance.
为了鉴定红树植物木榄耐盐性调控中的关键基因，对盐胁迫条件下侧根和主根进行了转录组分析。统计分析表明，在11997个基因中，分别有175个和403个基因在侧根和主根中表现出显著增加的高表达。在盐处理后的早期（1至12小时），两种类型的根中有160个基因上调。构建了28个选定的盐响应基因的表达载体并转化到根癌农杆菌中，然后筛选耐盐性。用脂质转移、锌指和锚蛋白重复蛋白基因转化的根癌农杆菌表现出增强的耐盐性。表达这三个基因的转基因拟南芥植物对NaCl的耐受性也有所提高。这些结果表明，农杆菌功能筛选是预筛选参与非生物胁迫耐受性基因的一种有效补充方法。
