Murakami Taro, Yamano Tetsuo, Nakama Akihiko, Mori Yoshiaki
Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
J AOAC Int. 2008 Nov-Dec;91(6):1402-8.
Single-laboratory method performance parameters were evaluated for analysis of biotin in total diet samples by hydrolyzed extraction and microbiological assay with Lactobacillus plantarum. The method was shown to be accurate, repeatable, rugged, and applicable for the determination of biotin in a broad range of matrixes and concentrations of total diet samples. The measurement uncertainty was evaluated with all food sample groups by combining the relative uncertainty of precision, recovery from each food group, and interday variation factors calculated from the ruggedness test results. The estimated daily intake of biotin in Osaka city and its expanded measurement uncertainty were 70.1 +/- 11.2 microg/day. The value was 1.6 times higher than the current adequate intake of biotin in Japan.
通过水解提取和植物乳杆菌微生物测定法，对单一实验室方法在总膳食样本中生物素分析的性能参数进行了评估。结果表明，该方法准确、可重复、耐用，适用于测定多种基质和不同浓度总膳食样本中的生物素。通过结合精密度相对不确定度、各食物组的回收率以及根据耐用性测试结果计算的日间变化因素，对所有食物样本组的测量不确定度进行了评估。大阪市生物素的估计每日摄入量及其扩展测量不确定度为70.1±11.2微克/天。该值比日本目前生物素的适宜摄入量高1.6倍。
