被动接触烟草烟雾的受试者的血液中血红蛋白和脂质过氧化代谢物水平、红细胞中的过氧化氢酶活性以及呼气峰值流速。

Levels of hemoglobin and lipid peroxidation metabolites in blood, catalase activity in erythrocytes and peak expiratory flow rate in subjects with passive exposure to tobacco smoke.

作者信息

Zawadzki Marcin, Gać Paweł, Poreba Rafał, Murawska-Ciałowicz Eugenia, Wielkoszyński Tomasz, Januszewska Lidia, Pawlas Krystyna, Andrzejak Ryszard

机构信息

Department of Hygiene, Wrocław Medical University, Wrocław, Poland.

出版信息

Pol Arch Med Wewn. 2008 Dec;118(12):705-12.

PMID:19202948

Abstract

INTRODUCTION

Exposure to tobacco smoke is an extremely important risk factor determining the development of respiratory diseases, cardiovascular diseases and cancer. Passive exposure is common and often not realized by the exposed subjects. Markers of tobacco smoke exposure are nicotine metabolites, i.e. cotinine and trans-3'-hydroxycotinine.

OBJECTIVES

The objective of the study was to assess the level of passive exposure to tobacco smoke among students and the exposure impact on the blood hemoglobin level, peak expiratory flow (PEF), lipid peroxidation level and antioxidant enzyme activity.

PATIENTS AND METHODS

A total of 104 subjects were enrolled in the study. The subjects were categorized in 3 subgroups depending on nicotine metabolite levels in blood (subgroup I with metabolite level > 100 ng/ml (high exposure); subgroup II with the metabolite level of 10-100 ng/ml; subgroup III with metabolite level <10 ng/ml). The blood hemoglobin level, PEF, levels of lipid peroxidation metabolites--malondialdehyde and 4-hydroxynonenal (MDA + 4-HNE) and catalase (CAT) activity were determined in all the subjects.

RESULTS

The study showed statistically significant differences in levels of lipid peroxidation metabolites and CAT activity. Levels of MDA + 4-HNE were higher in subgroup I than in subgroup II or III (I: 3.84 +/- 1.64 mmol/l; II: 2.25 +/- 0.94 mmol/l; III: 1.90 +/- 0.82 mmol/l; pI-II < 0.01; pI-III <0.001). CAT activity was statistically significantly lower in subgroup I than in subgroup III (I: 0.38 +/- 0.01 x 10(6) IU/g hemoglobin [Hb]; II: 0.38 +/- 0.03 x 10(6) IU/g Hb; III: 0.41 +/- 0.04 x 10(6) IU/g Hb; pI-III <0.05).

CONCLUSIONS

Passive exposure to tobacco smoke in the study population of students is common. The observed effects of passive exposure to tobacco smoke are similar to those of active smoking. It is postulated to undertake actions aiming at limiting passive exposure to tobacco smoke.

摘要

引言

接触烟草烟雾是决定呼吸系统疾病、心血管疾病和癌症发生的极其重要的风险因素。被动接触很常见，且接触者往往并未意识到。烟草烟雾接触的标志物是尼古丁代谢物，即可替宁和反式-3'-羟基可替宁。

目的

本研究的目的是评估学生中被动接触烟草烟雾的水平以及这种接触对血液血红蛋白水平、呼气峰值流速（PEF）、脂质过氧化水平和抗氧化酶活性的影响。

患者与方法

共有104名受试者参与本研究。根据血液中尼古丁代谢物水平将受试者分为3个亚组（I组代谢物水平>100 ng/ml（高接触）；II组代谢物水平为10 - 100 ng/ml；III组代谢物水平<10 ng/ml）。测定了所有受试者的血液血红蛋白水平、PEF、脂质过氧化代谢物——丙二醛和4-羟基壬烯醛（MDA + 4-HNE）的水平以及过氧化氢酶（CAT）活性。

结果

研究显示脂质过氧化代谢物水平和CAT活性存在统计学显著差异。I组MDA + 4-HNE水平高于II组或III组（I组：3.84±1.64 mmol/l；II组：2.25±0.94 mmol/l；III组：1.90±0.82 mmol/l；I-II组p<0.01；I-III组p<0.001）。I组CAT活性在统计学上显著低于III组（I组：0.38±0.01×10⁶ IU/g血红蛋白[Hb]；II组：0.38±0.03×10⁶ IU/g Hb；III组：0.41±0.04×10⁶ IU/g Hb；I-III组p<0.05）。