Campbell B K, Picton H M, Mann G E, McNeilly A S, Baird D T
University of Edinburgh Department of Obstetrics and Gynaecology, UK.
J Reprod Fertil. 1991 Sep;93(1):81-96. doi: 10.1530/jrf.0.0930081.
Treatment of ewes with steroid-free ovine follicular fluid (oFF) during the follicular phase of the oestrous cycle results in the immediate inhibition of the ovarian secretion of oestradiol, inhibin and androgens. An experiment was conducted to determine whether this effect of oFF was due to inhibin, or to direct inhibition of ovarian function by other factors in oFF. Eight ewes in which the left ovary and vascular pedicle had been autotransplanted to a site in the neck were studied during the breeding season. Luteal regression was induced in all animals by injection of cloprostenol (100 micrograms i.m.; PG) on Day 10 of the luteal phase. The animals were divided into two groups (n = 4) and treated with either steroid-free oFF (oFF; 3 ml s.c.; 3.2 microgram p1-26 alpha inhibin/ml) or steroid-free oFF in which the inhibin content had been reduced by greater than 90% (IFoFF; 3 ml s.c.; 0.3 microgram p1-26 alpha inhibin/ml) by affinity chromatography, 24 and 36 h after PG. Samples of ovarian and jugular venous blood were collected at (i) intervals of 4 h from 16 h before until 120 h after PG and (ii) intervals of 10 min from 48 to 52 h after injection of PG to investigate the pattern of pulsatile secretion of ovarian hormones. All ewes had previously been monitored during a normal PG-induced follicular phase. Injection of oFF resulted in an increase (P less than 0.05) in the concentration of inhibin in jugular venous plasma and a profound (P less than 0.001) and prolonged decrease in the peripheral concentration of follicle-stimulating hormone (FSH). Injection of IFoFF had no significant effect on peripheral concentrations of inhibin or FSH in the first 24 h after treatment; thereafter inhibin concentrations fell (P less than 0.01) progressively until 40 h and then increased (P less than 0.01) until 72 h after treatment. In both treatment groups, however, within 24-36 h of treatment the concentration of FSH increased 5-10-fold (P less than 0.001) to a peak that occurred within 48-60 h and then declined to basal concentrations within 72-84 h of treatment. The concentration of luteinizing hormone (LH) in jugular venous plasma increased in both groups after treatment (P less than 0.01), although the rise after injection of oFF only started after 24 h. Thereafter, there was a progressive increase in the concentration of LH, peaks occurring 48-60 h after treatment.(ABSTRACT TRUNCATED AT 400 WORDS)
在发情周期的卵泡期用无类固醇的绵羊卵泡液（oFF）处理母羊，会立即抑制卵巢分泌雌二醇、抑制素和雄激素。进行了一项实验，以确定oFF的这种作用是由于抑制素，还是由于oFF中其他因素对卵巢功能的直接抑制。在繁殖季节，对8只将左卵巢和血管蒂自体移植到颈部某一部位的母羊进行了研究。在黄体期第10天，给所有动物注射氯前列醇（100微克，肌肉注射；PG）以诱导黄体退化。将动物分为两组（n = 4），在注射PG后24小时和36小时，分别用无类固醇的oFF（oFF；3毫升，皮下注射；3.2微克p1 - 26α抑制素/毫升）或通过亲和层析使抑制素含量降低超过90%的无类固醇oFF（IFoFF；3毫升，皮下注射；0.3微克p1 - 26α抑制素/毫升）进行处理。在（i）从PG前16小时到PG后120小时每隔4小时以及（ii）在注射PG后48至52小时每隔10分钟采集卵巢和颈静脉血样，以研究卵巢激素的脉冲式分泌模式。所有母羊此前在正常PG诱导的卵泡期都已接受监测。注射oFF导致颈静脉血浆中抑制素浓度升高（P < 0.05），促卵泡生成素（FSH）外周浓度显著（P < 0.001）且持续下降。注射IFoFF在处理后的前24小时对抑制素或FSH的外周浓度无显著影响；此后抑制素浓度逐渐下降（P < 0.01）直至40小时，然后在处理后72小时内升高（P < 0.01）。然而，在两个处理组中，处理后24 - 36小时内FSH浓度升高5 - 10倍（P < 0.001），在48 - 60小时达到峰值，然后在处理后72 - 84小时降至基础浓度。处理后两组颈静脉血浆中促黄体生成素（LH）浓度均升高（P < 0.01），尽管注射oFF后的升高在24小时后才开始。此后，LH浓度逐渐升高，在处理后48 - 60小时出现峰值。（摘要截断于400字）
