[微小RNA-18与BTG2在肝细胞癌发生中的关系]
[The relationship between microRNA-18 and BTG2 in the carcinogenesis of hepatocellular carcinoma].
作者信息
Li Qiong, Wang Ge, Zhang Zhi-Min
机构信息
Cancer Center, Institute of Surgery Research, Daping Hospital, Third Minitary Medical University, Chongqing 400042, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2009 Jan;17(1):42-5.
OBJECTIVE
To study the difference of microRNA expression between HepG2 cells and L02 cells, and to identify the target genes of microRNA-18 (miR-18).
METHODS
The differentially expressed miRNAs between HepG2 cells and L02 cells were identified by miRNA chip. Target genes of miR-18 were predicted bioinformatically. Furthermore, the expression of B-cell translocation gene 2 (BTG2), a putative target gene of miR-18, was analyzed in hepatocellular carcinoma tissues and the surrounding non-cancerous tissues by RT-PCR and northern blot.
RESULTS
miR-18 was over-expressed in HepG2 cells compared to L02 cells. Altogether 609 genes, including genes involved in cell proliferation, differentiation, apoptosis and transcriptional regulation, are identified as putative miR-18 targets. The mRNA level of BTG2 was much lower in hepatocellular carcinoma tissues than in the corresponding non-cancerous tissues.
CONCLUSION
miR-18 is over-expressed in HepG2 cells compared to L02 cells, and it may negatively regulate the expression of BTG2, a tumor suppressor gene.
目的
研究HepG2细胞和L02细胞之间微小RNA表达的差异，并鉴定微小RNA-18（miR-18）的靶基因。
方法
通过微小RNA芯片鉴定HepG2细胞和L02细胞之间差异表达的微小RNA。利用生物信息学方法预测miR-18的靶基因。此外，采用逆转录聚合酶链反应（RT-PCR）和Northern印迹法分析miR-18的假定靶基因B细胞易位基因2（BTG2）在肝癌组织及其周围非癌组织中的表达。
结果
与L02细胞相比，miR-18在HepG2细胞中过表达。共鉴定出609个基因作为假定的miR-18靶标，这些基因涉及细胞增殖、分化、凋亡和转录调控。BTG2的mRNA水平在肝癌组织中比在相应的非癌组织中低得多。
结论
与L02细胞相比，miR-18在HepG2细胞中过表达，并且它可能负向调节肿瘤抑制基因BTG2的表达。
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