使用新型固定相对有机酸进行高效液相色谱分析。

HPLC analysis of organic acids using a novel stationary phase.

作者信息

de Quirós A Rodríguez-Bernaldo, Lage-Yusty M A, López-Hernández J

机构信息

Analytical Chemistry, Nutrition and Bromatology Department, Pharmacy Faculty, Campus Sur s/n, University of Santiago de Compostela, 15782 Santiago de Compostela, La Coruña, Spain.

出版信息

Talanta. 2009 Apr 30;78(2):643-6. doi: 10.1016/j.talanta.2008.11.013. Epub 2008 Nov 21.

DOI:10.1016/j.talanta.2008.11.013

PMID:19203638

Abstract

In the present work, a high performance liquid chromatographic method with UV detection for the separation of six organic acids including, tartaric, malic, acetic, lactic, citric and succinic is described. The separation was performed on a novel stationary phase TEKNOKROMA, Tr-010065 Mediterranea sea(18) (15 cm x 0.4 cm, i.d. 3 microm) and using water with a 0.1% (v/v) of formic acid as mobile phase. The advantages of this packing over a conventional octadecylsilane (ODS2) column are reported. The method was validated with respect to linearity, limits of detection and repeatabilities within day and between days and satisfactory results were obtained. The proposed method was applied for the determination of these compounds in commercially available white wines. The samples were injected directly without previous treatment. LC-MS was used as a confirmatory technique.

摘要

在本研究中，描述了一种采用紫外检测的高效液相色谱法，用于分离酒石酸、苹果酸、乙酸、乳酸、柠檬酸和琥珀酸六种有机酸。分离在新型固定相TEKNOKROMA、Tr-010065地中海海相（18）（15 cm×0.4 cm，内径3 µm）上进行，以含有0.1%（v/v）甲酸的水作为流动相。报道了这种填料相对于传统十八烷基硅烷（ODS2）柱的优势。该方法在线性、检测限以及日内和日间重复性方面进行了验证，并获得了满意的结果。所提出的方法用于测定市售白葡萄酒中这些化合物的含量。样品无需预先处理即可直接进样。采用液相色谱-质谱联用作为确证技术。