金丝桃（Hypericum thasium Griseb.）的细胞活性氧抑制成分

Cellular reactive oxygen species inhibitory constituents of Hypericum thasium Griseb.

作者信息

Demirkiran Ozlem, Ahmed Mesaik M, Beynek Hayrettin, Abbaskhan Ahmed, Iqbal Choudhary M

机构信息

Department of Chemistry, Faculty of Science and Arts, Trakya University, Edirne 22030, Turkey.

出版信息

Phytochemistry. 2009 Jan;70(2):244-9. doi: 10.1016/j.phytochem.2008.10.006. Epub 2009 Feb 7.

DOI:10.1016/j.phytochem.2008.10.006

PMID:19203769

Abstract

The phytochemical investigation of the ethyl acetate extract of Hypericum thasium has led to the characterization of four benzophenone derivatives 1-4, a known benzophenone 5 and four known flavonoids, quercetin (6), quercitrin (7), isoquercetin (8), and 3, 8''-biapigenin (9). Lucigenin- and luminal-based chemiluminescence assays were employed to monitor the inhibitory activity of these compounds towards the production of reactive oxygen species (ROS) by human polymorphoneutrophils (PMNs). The assay results showed that benzophenones 1 and 3 are extracellular inhibitors of ROS production, while flavonoids 6, 8, and 9 can modulate intracellular ROS production.

摘要

对金丝桃属植物（Hypericum thasium）乙酸乙酯提取物的植物化学研究已鉴定出四种二苯甲酮衍生物1-4、一种已知的二苯甲酮5以及四种已知的黄酮类化合物，即槲皮素（6）、槲皮苷（7）、异槲皮苷（8）和3,8''-双芹菜素（9）。采用基于光泽精和鲁米诺的化学发光测定法监测这些化合物对人多形核中性粒细胞（PMN）产生活性氧（ROS）的抑制活性。测定结果表明，二苯甲酮1和3是细胞外ROS产生抑制剂，而黄酮类化合物6、8和9可调节细胞内ROS的产生。