Kim Hyunmyung, Choi Youngjin, Lim Jun, Paik Seung-R, Jung Seunho
Department of Bioscience and Biotechnology and Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, South Korea.
Chirality. 2009 Nov;21(10):937-42. doi: 10.1002/chir.20696.
The chiral separation of (+/-)-catechin was investigated by capillary electrophoresis using characterized succinyl-beta-cyclodextrins (Suc-beta-CDs) with one to three degree of substitution values. The effects of nature and concentration of Suc-beta-CDs and running buffer pH on the migration time and resolution of (+/-)-catechin are discussed. All three kinds of Suc-beta-CDs show a clear baseline separation of (+/-)-catechin in capillary electrophoresis. Mono-Suc-beta-CD effectively separated (+/-)-catechin, and additional substituted CDs (di- and tri-Suc-beta-CD) were capable of chiral separation at a broad pH range. The optimum running conditions were found to be 100 mM borate buffer (pH 9.8) containing 5 mM mono-Suc-beta-CD with no methanol organic modifier.
使用具有一至三个取代度值的特征化琥珀酰-β-环糊精（Suc-β-CD），通过毛细管电泳研究了（±）-儿茶素的手性分离。讨论了Suc-β-CD的性质和浓度以及运行缓冲液pH对（±）-儿茶素迁移时间和分离度的影响。所有三种Suc-β-CD在毛细管电泳中均显示出（±）-儿茶素的清晰基线分离。单琥珀酰-β-环糊精有效地分离了（±）-儿茶素，另外的取代环糊精（二琥珀酰-β-环糊精和三琥珀酰-β-环糊精）能够在较宽的pH范围内进行手性分离。发现最佳运行条件为含有5 mM单琥珀酰-β-环糊精且无甲醇有机改性剂的100 mM硼酸盐缓冲液（pH 9.8）。
