Megías Cristina, Pedroche Justo, Del Mar Yust María, Alaiz Manuel, Girón-Calle Julio, Millán Francisco, Vioque Javier
Instituto de la Grasa (C.S.I.C.), Padre García Tejero 4, Sevilla 41012, Spain.
Plant Foods Hum Nutr. 2009 Jun;64(2):86-93. doi: 10.1007/s11130-009-0108-1.
Plant protein hydrolysates are a source of bioactive peptides. There are peptides that decrease the micellar cholesterol solubility from bile acids and therefore may reduce in vivo cholesterol absorption. The presence of these peptides in sunflower protein hydrolysates has been studied. Sunflower protein hydrolysates produced with alcalase plus flavourzyme or with pepsin plus pancreatin inhibited in some degree the cholesterol incorporation to micelles. Protein hydrolysates generated after 30 min of hydrolysis with alcalase, and after 30 min of hydrolysis with pepsin, were the inhibitoriest of the cholesterol incorporation to micelles. The average amino acid hydrophobicity of inhibitory peptides in cholesterol micelles was higher than the observed in the corresponding protein hydrolysates. This high hydrophobicity probably favours their inclusion in the lipid micelles. In vivo, this inhibition may translate in a decrease of cholesterol absorption. Reported results show that a combination of different characteristics such as peptide size or hydrophobicity may be responsible of the inhibitory activity of generated peptides.
植物蛋白水解物是生物活性肽的一个来源。有些肽会降低胆汁酸中胶束胆固醇的溶解度，因此可能会减少体内胆固醇的吸收。人们已经对向日葵蛋白水解物中这些肽的存在情况进行了研究。用碱性蛋白酶加风味酶或用胃蛋白酶加胰酶生产的向日葵蛋白水解物在一定程度上抑制了胆固醇掺入胶束。用碱性蛋白酶水解30分钟后以及用胃蛋白酶水解30分钟后产生的蛋白水解物对胆固醇掺入胶束的抑制作用最强。胆固醇胶束中抑制性肽的平均氨基酸疏水性高于相应蛋白水解物中的观察值。这种高疏水性可能有利于它们融入脂质胶束。在体内，这种抑制作用可能会转化为胆固醇吸收的减少。报告结果表明，肽大小或疏水性等不同特性的组合可能是所产生肽的抑制活性的原因。
