Yelinek Jordan T, He Cynthia Y, Warren Graham
Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
Traffic. 2009 Mar;10(3):300-6. doi: 10.1111/j.1600-0854.2008.00873.x.
Golgi duplication in the protozoan parasite Trypanosoma brucei has been tracked using serial thin section three-dimensional reconstructions of transmission electron micrographs. The old Golgi maintains a constant size (approximately 0.060 microm(3)) throughout the cell cycle. A morphologically identifiable new Golgi appears at approximately 0.20 of the cell cycle (defined by the size of the nucleus and lasting about 9 h) and grows from approximately 0.018 microm(3) until it is the same size as the old Golgi (by approximately 0.55 of the cell cycle). Morphologically identifiable late Golgi appear at approximately 0.58 of the cell cycle, but their volume ( approximately 0.036 microm(3)) did not change significantly. Cryoimmunoelectron microscopy was used to identify candidates for the earliest new Golgi structures, and these comprised clusters of vesicles containing Golgi reassembly stacking protein (GRASP) near an endoplasmic reticulum exit site. These results, combined with earlier fluorescence data, suggest that the new Golgi begins functioning before cisternal stacks are formed.
利用透射电子显微镜的连续超薄切片三维重建技术，对原生动物寄生虫布氏锥虫中的高尔基体复制过程进行了追踪。在整个细胞周期中，旧的高尔基体保持恒定大小（约0.060立方微米）。在细胞周期约0.20时（由细胞核大小定义，持续约9小时）出现形态上可识别的新高尔基体，其从约0.018立方微米开始生长，直至与旧高尔基体大小相同（细胞周期约0.55时）。形态上可识别的晚期高尔基体在细胞周期约0.58时出现，但其体积（约0.036立方微米）没有显著变化。冷冻免疫电子显微镜用于鉴定最早的新高尔基体结构的候选物，这些候选物包括在内质网出口位点附近含有高尔基体重新组装堆叠蛋白（GRASP）的囊泡簇。这些结果与早期的荧光数据相结合，表明新高尔基体在形成扁平囊堆叠之前就开始发挥功能。
