Laboratory of Parasitology CHU Farhat Hached, Sousse, Tunisia.
Mycoses. 2010 Jan;53(1):72-7. doi: 10.1111/j.1439-0507.2008.01664.x. Epub 2009 Jan 14.
Candida albicans has become an important cause of nosocomial infections in neonatal intensive care units (NICUs). The aim of the present study was to compare C. albicans strains isolated from neonates (NN) suffering from systemic candidosis and from nurses in order to determine the relatedness between NN and health workers' strains. Thirty-one C. albicans strains were isolated from 18 NN admitted to the NICU of the neonatology service of Farhat Hached Hospital of Sousse, Tunisia and suffering from systemic candidosis, together with five strains recovered from nurses suffering from C. albicans onychomycosis. Two additional strains were tested, one from an adult patient who developed a systemic candidosis and the second from an adult with inguinal intertrigo. All strains were karyotyped by pulsed-field gel electrophoresis (PFGE) with a CHEF-DR II system. Analysis of PFGE patterns yielded by the 38 strains tested led to the identification of three pulsotypes that were designated I, II and III, and consisted of six chromosomal bands with a size ranging from 700 to >2500 kbp. The most widespread was the pulsotype I, which was shared by 17 NN and the five nurses' strains. The identity between NN and nurses' strains is very suggestive of a nosocomial acquisition from health-workers.
白色念珠菌已成为新生儿重症监护病房（NICU）医院感染的重要原因。本研究旨在比较从患有全身性念珠菌病的新生儿（NN）和护士中分离出的白色念珠菌菌株，以确定 NN 与卫生工作者菌株之间的相关性。从突尼斯苏塞法哈特哈奇医院新生儿科 NICU 收治的 18 名患有全身性念珠菌病的 NN 中分离出 31 株白色念珠菌，以及从患有白色念珠菌甲真菌病的 5 名护士中分离出的 5 株。还测试了另外两个菌株，一个来自患有全身性念珠菌病的成年患者，第二个来自腹股沟间擦疹的成年患者。所有菌株均通过脉冲场凝胶电泳（PFGE）用 CHEF-DR II 系统进行核型分析。对 38 株测试菌株的 PFGE 图谱分析导致鉴定出三种脉冲型，分别为 I、II 和 III，由 6 个大小在 700 到>2500 kbp 之间的染色体带组成。最广泛的是脉冲型 I，17 名 NN 和 5 名护士的菌株均为该型。NN 和护士菌株之间的同一性非常提示从卫生工作者那里获得了医院感染。
