Rudman D, Hollins B M, Lewis N C, Scott J W
Am J Physiol. 1977 Apr;232(4):E353-7. doi: 10.1152/ajpendo.1977.232.4.E353.
Choroid plexus of rabbit and rat was incubated for 2-30 min at 37 degrees C under 95% O2-5% CO2 in Tyrode solution containing 10 mM glucose and 1 mM theophylline with these agents: epinephrine, norepinephrine, isoproterenol, dopamine, histamine, serotonin, arginine, and lysine vasopressins, oxytocin, angiotensin, adrenocorticotropin (ACTH), beta-melanocyte-stimulating hormone, and choroid plexus peptide IIF. After incubation, tissue and medium were analyzed for 3', 5' -cyclic adenosine monophosphate (cAMP) content. Each amine or peptide was tested initially at 1,000 microng/ml. Only ACTH and serotonin affected cAMP content of rabbit choroid plexus. At 1,000 microng/ml, these agents caused a 10 and 4 times (respectively) increase in cAMP content of tissue + medium at 2-10 min with decline in content at 10-30 min. More than 90% of the increment was located in tissue, less than 10% in medium. Minimal effective dose (MED) to cause a significant (P less than .05) accumulation of cAMP was 0.1 microng/ml (2.2 x 10(-8) M) for ACTH and 10 microng/ml (5.7 x10(-3) M) for serotonin. Only isoproterenol, epinephrine, and norepinephrine influenced cAMP content of rat choroid plexus. MED's for this effect by isoproterenol, epinephrine, and norepinephrine were .001, .01, and 10 microng/ml (4.7 x 10(-9), 5.5 x 10(-8), and 5.9 x 10(-5) M), respectively.
将兔和大鼠的脉络丛在含10 mM葡萄糖和1 mM茶碱的台氏液中，于37℃、95% O₂ - 5% CO₂条件下孵育2 - 30分钟，所用试剂如下：肾上腺素、去甲肾上腺素、异丙肾上腺素、多巴胺、组胺、5 - 羟色胺、精氨酸和赖氨酸加压素、催产素、血管紧张素、促肾上腺皮质激素（ACTH）、β - 黑素细胞刺激素以及脉络丛肽IIF。孵育后，对组织和培养基进行3',5' - 环磷酸腺苷（cAMP）含量分析。每种胺或肽最初均以1000微克/毫升进行测试。只有ACTH和5 - 羟色胺影响兔脉络丛的cAMP含量。在1000微克/毫升时，这些试剂在2 - 10分钟内使组织 + 培养基中的cAMP含量分别增加了10倍和4倍，而在10 - 30分钟时含量下降。增加量的90%以上位于组织中，不到10%在培养基中。引起cAMP显著（P < 0.05）积累的最小有效剂量（MED），ACTH为0.1微克/毫升（2.2×10⁻⁸ M），5 - 羟色胺为10微克/毫升（5.7×10⁻³ M）。只有异丙肾上腺素、肾上腺素和去甲肾上腺素影响大鼠脉络丛的cAMP含量。异丙肾上腺素、肾上腺素和去甲肾上腺素产生此效应的MED分别为0.001、0.01和10微克/毫升（4.7×10⁻⁹、5.5×10⁻⁸和5.9×10⁻⁵ M）。
