Yang Ronghua, Jin Jianyu, Long Liping, Wang Yongxiang, Wang Hao, Tan Weihong
State Key Lab of Chemo/Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Changsa, 410082, China.
Chem Commun (Camb). 2009 Jan 21(3):322-4. doi: 10.1039/b816638b. Epub 2008 Nov 20.
We report that the hydrogen-bonding pattern in a molecular beacon can be replaced by metal-dependent pairs of Hg(2+) and DNA thymine (T) bases. A molecular beacon based on T-Hg(2+)-T exhibits a lower background signal and higher thermostability than regular molecular beacons.
我们报告称，分子信标中的氢键模式可被汞离子（Hg(2+)）与DNA胸腺嘧啶（T）碱基形成的金属依赖性配对所取代。基于T-Hg(2+)-T的分子信标比常规分子信标具有更低的背景信号和更高的热稳定性。
