埃及血吸虫病相关膀胱癌患者谷胱甘肽S-转移酶（GST-M1和GST-T1）的基因多态性

Genetic polymorphism of glutathione-S-transferase (GST-M1 and GST-T1) in schistosomiasis -associated bladder cancer in Egyptian patients.

作者信息

El Nouby Kholoud A, Abd El Hameed Amal H, Negm Osama E, Hamouda Hala E, El Gamal Osama M, Ismail Ghada M

机构信息

Department of Parasitology, Faculty of Medicine, Tanta University, Tanta, Egypt.

出版信息

J Egypt Soc Parasitol. 2008 Dec;38(3):991-1006.

PMID:19209780

Abstract

This work was carried out on three groups, 30 Egyptian patients with Schistosoma haematobium (S. haematobium) with bladder cancer (15), and without bladder cancer (15), as well as 15 normal individuals as a control. All the individuals were subjected to measurement of serum level of GST by using ELISA technique and genotyping for GST-M1 & GST-T1 using PCR technique. The results proved that GST serum level was significantly deceased in S. haematobium patients with bladder cancer as compared to the other groups. The PCR results for the GST-M1 & GST-T1 genotyping showed 4 categories, (M1+ve/T1+ve, M1+ve/T1-ve, M1-ve/T1+ve, M1-ve/T1/-ve). There was a significant decrease in enzyme levels in patients with GST-M1-ve/T1-ve as compared to the other categories. Besides, there was a significant increased risk for bladder cancer development in patients with combined gene deletion (OR = 40) which represented mainly in S. haematobium patients with bladder cancer (53.3% = M1-ve/TI-ve).

摘要

这项研究针对三组对象展开，30名患有埃及血吸虫病（埃及血吸虫）的埃及患者，其中15名患有膀胱癌，15名未患膀胱癌，还有15名正常个体作为对照。所有个体均采用ELISA技术检测血清谷胱甘肽S-转移酶（GST）水平，并使用PCR技术对GST-M1和GST-T1进行基因分型。结果证明，与其他组相比，患有膀胱癌的埃及血吸虫病患者的血清GST水平显著降低。GST-M1和GST-T1基因分型的PCR结果显示有4种类型，即（M1阳性/T1阳性、M1阳性/T1阴性、M1阴性/T1阳性、M1阴性/T1阴性）。与其他类型相比，GST-M1阴性/T1阴性患者的酶水平显著降低。此外，联合基因缺失患者患膀胱癌的风险显著增加（比值比=40），这主要体现在患有膀胱癌的埃及血吸虫病患者中（53.3%=M1阴性/T1阴性）。