Department of Psychiatry, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7250, USA.
J Neuroendocrinol. 1989 Aug 1;1(4):273-8. doi: 10.1111/j.1365-2826.1989.tb00115.x.
Abstract Estrogen has numerous effects on immunoreactive levels of oxytocin (OXT) centrally, particularly in the preoptic lateral subcommissural nucleus (LSN). In this study in situ hybridization of a 38-base oligodeoxyribonucleotide (38mer) complementary to OXT mRNA revealed that estrogen treatment altered the pattern of OXT production in the rostral LSN and the more caudal anterior commissural nucleus. Rats were injected with 20 ng estradiol benzoate or sesame oil vehicle im 4 and 5 days after ovariectomy. On the sixth day all animals were perfused with paraformaldehyde-glutaraldehyde and their brains sectioned to 10 mum thickness in a -10 degrees C cryostat. Coronal brain sections were taken from four parallel levels of the preoptic-anterior hypothalamic area. These sections were mounted and hybridized in situ to a [l(125)]-labeled 38mer for 16 h at 37 degrees C. Washed and dried slides were processed for autoradiography and analyzed with a light microscope. The effect of estrogen on OXT production differed between the rostral and caudal sections in both the LSN and periventricular (PeV) areas. Estrogen significantly increased OXT mRNA levels in LSN cells while decreasing hybridization in the anterior commissural nucleus cells. Changes in frequency patterns in the PeV paralleled those in the LSN with a significant drop of hybridization in the caudal PeV. Neurons hybridizing 38mer probe were also found in several other areas including the ventral medial preoptic area, lateral hypothalamus, bed nucleus of the stria terminalis and the nucleus triangularis septi. OXT mRNA levels were affected by estrogen treatments and this effect differed between the preoptic area and anterior hypothalamus. The sensitivity of LSN oxytocinergic cells to estrogen has implications for estrogen-sensitive OXT-enhanced reproductive behaviors.
摘要 雌激素对中枢的催产素（OXT）免疫反应水平有许多影响，特别是在下丘脑前外侧亚室旁核（LSN）。在这项研究中，用 38 个碱基的寡脱氧核苷酸（38mer）对 OXT mRNA 进行原位杂交，结果显示雌激素处理改变了 LSN 前部和更靠后的前连合核中 OXT 的产生模式。大鼠在卵巢切除后第 4 天和第 5 天每天注射 20ng 苯甲酸雌二醇或芝麻油溶剂。第六天，所有动物都用多聚甲醛-戊二醛灌注，然后在-10°C 冷冻切片机中切成 10 微米厚的切片。从前下丘脑视前区的四个平行水平取冠状脑切片。这些切片在原位与 [l(125)]标记的 38mer 杂交 16 小时，在 37°C 下进行。将洗涤和干燥的载玻片进行放射自显影处理，并在光学显微镜下进行分析。雌激素对 OXT 产生的影响在 LSN 和脑室周围（PeV）区域的前脑和后脑切片之间有所不同。雌激素显著增加了 LSN 细胞中的 OXT mRNA 水平，同时减少了前连合核细胞中的杂交。PeV 中的频率模式变化与 LSN 平行，尾部 PeV 中的杂交显著下降。在几个其他区域也发现了与 38mer 探针杂交的神经元，包括腹内侧视前区、下丘脑外侧区、终纹床核和隔三角核。OXT mRNA 水平受到雌激素处理的影响，这种影响在前脑和下丘脑前部之间有所不同。LSN 催产素能细胞对雌激素的敏感性对雌激素敏感的 OXT 增强的生殖行为具有重要意义。
