大鼠垂体中激肽释放酶基因家族的表达：雌激素的作用及神经垂体中叶中另一个家族成员的表达。

The expression of the kallikrein gene family in the rat pituitary: oestrogen effects and the expression of an additional family member in the neurointermediate lobe.

机构信息

Medical Research Centre, Prince Henry's Hospital, Melbourne, Australia 3004.

出版信息

J Neuroendocrinol. 1989 Jun 1;1(3):198-203. doi: 10.1111/j.1365-2826.1989.tb00103.x.

DOI:10.1111/j.1365-2826.1989.tb00103.x

PMID:19210455

Abstract

Abstract Using a series of oligonucleotide probes (18 to 21 mers) specific for members of the rat kallikrein/tonin gene family (PS, S1, S2, S3, K1, P1), we have shown by Northern blot analysis that the oestrogen-dependent kallikrein gene expressed in the male and female rat anterior pituitary is true kallikrein (PS). In addition, we have demonstrated that oestrogen treatment may also induce PS gene expression in the male and female rat neurointermediate lobe. None of the other five rat arginyl-esteropeptidase genes so far described (S1, S2, S3, K1, P1) was found to be expressed in the anterior pituitary or neurointermediate lobe under these conditions. However, the demonstration of an additional hybridization signal in the male neurointermediate lobe using a relatively non-specific PS gene probe suggests the expression of another, as yet uncharacterized, kallikrein gene family member in this tissue.

摘要

摘要我们使用一系列针对大鼠激肽释放酶/豚鼠抑肽基因家族（PS、S1、S2、S3、K1、P1）成员的寡核苷酸探针（18-21 个碱基），通过 Northern blot 分析表明，在雄性和雌性大鼠垂体前叶中表达的雌激素依赖性激肽释放酶基因是真正的激肽释放酶（PS）。此外，我们还证明，雌激素处理也可能诱导雄性和雌性大鼠神经中间叶中 PS 基因的表达。迄今为止，在这些条件下，尚未发现其他五种大鼠精氨酸酯肽酶基因（S1、S2、S3、K1、P1）在垂体前叶或神经中间叶中表达。然而，使用相对非特异性的 PS 基因探针在雄性神经中间叶中检测到另一个杂交信号，表明在该组织中表达另一种尚未确定的激肽释放酶基因家族成员。