Salo J, Kaigle Holm A, Indahl A, Mackiewicz Z, Sukura A, Holm S, Jämsen E, Konttinen Y T
Department of Orthopaedic Surgery, Helsinki University Central Hospital, Helsinki, Finland.
Clin Exp Rheumatol. 2008 Nov-Dec;26(6):1018-26.
To analyze immunohistochemically the localization of the VEGF receptors in experimental intervertebral disc degeneration tissues in a pig model.
In six domestic pigs, the cranial bony endplate of the L4 vertebra were perforated into the nucleus pulposus. Three months postoperatively, the animals were sacrificed and the experimental and control vertebrae, complete with intervertebral discs, were excised and subjected for immunohistochemical staining of vascular endothelial growth factor receptors (VEGFR) along with VEGF - A, -C, -D and blood and lymphatic vessel markers vWF and LYVE-1.
The results of immunohistochemical analysis of experimental samples showed VEGFR-1 (Flt-1) expression in intervertebral disc and all paradiscal tissues studied. In control samples expression of VEGFR-1 was lower and absent in the intervertebral discs. Comparatively less of VEGFR-2 (KDR/Flk-1) and VEGFR-3 (Flt-4) than VEGFR-1was found in degenerated intervertebral discs and paradiscal tissues. In contiguous control intervertebral discs and control paradiscal tissues VEGFR-2 and-3 receptors were expressed to a lower extent than in experimental tissues or were even totally absent. Also growth factors VEGF-A, -C, -D, as well as von Willebrand factor and to a much lower extent LYVE-1 were differently expressed in experimental and control intervertebral discs.
In experimental intervertebral disc degeneration, VEGF receptors were expressed in the damaged disc and paradiscal tissues. In the same tissues, VEGF-A, -C, and -D, signs of blood and lymphatic vessel in-growth and reactive/adaptive vertebral bone remodelling were found.
通过免疫组织化学方法分析猪模型实验性椎间盘退变组织中血管内皮生长因子（VEGF）受体的定位。
选取6头家猪，在L4椎体的颅侧骨终板打孔至髓核。术后3个月，处死动物，切除实验椎骨和对照椎骨及其完整椎间盘，进行血管内皮生长因子受体（VEGFR）以及VEGF - A、-C、-D与血管和淋巴管标志物血管性血友病因子（vWF）和淋巴管内皮透明质酸受体-1（LYVE-1）的免疫组织化学染色。
实验样本的免疫组织化学分析结果显示，VEGFR-1（Flt-1）在研究的椎间盘及所有椎间盘旁组织中均有表达。对照样本中，VEGFR-1在椎间盘中表达较低或无表达。在退变的椎间盘和椎间盘旁组织中发现，VEGFR-2（KDR/Flk-1）和VEGFR-3（Flt-4）的表达量比VEGFR-1少。在相邻的对照椎间盘和对照椎间盘旁组织中，VEGFR-2和-3受体的表达程度低于实验组织，甚至完全缺失。此外，生长因子VEGF-A、-C、-D以及血管性血友病因子，还有表达程度低得多的LYVE-1，在实验性和对照性椎间盘中的表达也存在差异。
在实验性椎间盘退变中，VEGF受体在受损的椎间盘和椎间盘旁组织中表达。在相同组织中，发现了VEGF-A、-C和-D、血管和淋巴管长入迹象以及反应性/适应性椎体骨重塑。
