Wang G H, Xue C Y, Chen F, Ma Y L, Zhang X B, Bi Y Z, Cao Y C
College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China.
Poult Sci. 2009 Mar;88(3):504-10. doi: 10.3382/ps.2008-00329.
The study was to investigate the effects of combinations of ochratoxin A (OTA) and T-2 toxin on immune function of yellow-feathered broiler chickens. Three-hundred sixty 21-d-old broiler chickens were randomly assigned to 3 groups, each group consisting of 4 duplicates each with 30 chickens. The 3 groups were fed the following diets for 3 wk: C, basal diet (control, mycotoxin-free); L, basal diet + 0.25 mg/kg of OTA, 0.5 mg/kg of T-2 toxin; and H, basal diet + 0.5 mg/kg of OTA, 1 mg/kg of T-2 toxin. Body weight and feed consumption of chickens in the H group decreased significantly (P < 0.05) during the study, but their efficiency of feed utilization was not affected. The feeding of OTA-T-2 toxin diets decreased not only the relative weight of spleen, thymus, and bursa of Fabricius, but also serum concentrations of total protein, albumin, and globulin. Meanwhile, the feeding of OTA-T-2 toxin diets elevated the activities of serum gamma-glutamyltransferase, asparate aminotransferase, and alanine aminotransferase. The results of methyl thiazolyl tetrazolium reduction assay indicated that the mitogenic responses of peripheral blood lymphocytes were diminished significantly (P < 0.05 for L group; P < 0.01 for H group). Flow cytometry was employed to determine 3 indexes in peripheral blood lymphocyte of broilers, including CD4(+)/CD3(+), CD8(+)/CD3(+), and CD4(+)/CD8(+). Both toxin treatments significantly decreased (P < 0.01) CD4(+)/CD3(+) and CD4(+)/CD8(+) ratios. In summary, the combination of OTA and T-2 toxin impaired chick immune function even at combined concentrations as low as 0.25 mg/kg of OTA and 0.5 mg/kg of T-2 toxin.
本研究旨在探讨赭曲霉毒素A（OTA）与T-2毒素联合作用对黄羽肉鸡免疫功能的影响。将360只21日龄的肉鸡随机分为3组，每组4个重复，每个重复30只鸡。3组鸡分别饲喂以下日粮3周：C组，基础日粮（对照，无霉菌毒素）；L组，基础日粮+0.25 mg/kg OTA、0.5 mg/kg T-2毒素；H组，基础日粮+0.5 mg/kg OTA、1 mg/kg T-2毒素。在试验期间，H组鸡的体重和采食量显著下降（P<0.05），但其饲料利用率未受影响。饲喂OTA-T-2毒素日粮不仅降低了脾脏、胸腺和法氏囊的相对重量，还降低了血清总蛋白、白蛋白和球蛋白的浓度。同时，饲喂OTA-T-2毒素日粮提高了血清γ-谷氨酰转移酶、天冬氨酸转氨酶和丙氨酸转氨酶的活性。噻唑蓝还原试验结果表明，外周血淋巴细胞的促有丝分裂反应显著降低（L组P<0.05；H组P<0.01）。采用流式细胞术检测肉鸡外周血淋巴细胞的3个指标，包括CD4(+)/CD3(+)、CD8(+)/CD3(+)和CD4(+)/CD8(+)。两种毒素处理均显著降低了（P<0.01）CD4(+)/CD3(+)和CD4(+)/CD8(+)比值。综上所述，即使OTA和T-2毒素的联合浓度低至0.25 mg/kg OTA和0.5 mg/kg T-2毒素，其组合仍会损害雏鸡的免疫功能。
