van Zutphen L F, Den Bieman M, Lankhorst A, Demant P
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Lab Anim. 1991 Jul;25(3):193-7. doi: 10.1258/002367791780808329.
Recombinant congenic strains (RCS) constitute a set of inbred strains which are designed to dissect the genetic control of multigenic traits, such as tumour susceptibility or disease resistance. Each RCS contains a small fraction of the genome of a common donor strain, while the majority of genes stem from a common background strain. We tested at two stages of the inbreeding process in 20 RCS, derived from BALB/cHeA and STS/A, to see whether alleles from the STS/A donor strain are distributed over the RCS in a ratio as would theoretically be expected. Four marker genes (Pep-3; Pgm-1; Gpi-1 and Es-3) located at 4 different chromosomes were selected and the allelic distribution was tested after 3-4 and after 12 generations of inbreeding. The data obtained do not significantly deviate from the expected pattern, thus supporting the validity of the concept of RCS.
重组近交系（RCS）是一组近交系，旨在剖析多基因性状（如肿瘤易感性或抗病性）的遗传控制。每个RCS包含一小部分共同供体品系的基因组，而大多数基因则来自共同的背景品系。我们在20个源自BALB/cHeA和STS/A的RCS的近交过程的两个阶段进行了测试，以查看来自STS/A供体品系的等位基因是否以理论预期的比例分布在RCS中。选择了位于4条不同染色体上的4个标记基因（Pep-3；Pgm-1；Gpi-1和Es-3），并在近交3-4代和12代后测试了等位基因分布。获得的数据与预期模式没有显著偏差，从而支持了RCS概念的有效性。
