[降浊合剂对味精诱导肥胖大鼠糖脂代谢的影响]

[Influence of jiangzhuo mixture on glucose and lipid metabolism in MSG obese-induced rats].

作者信息

Zhou Kai, Gong Wen-Bo, Zhou Jian-Yang

机构信息

Ningbo Traditional Chinese Medicine Hospital, Zhejiang.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Nov;28(11):1014-7.

PMID:19213346

Abstract

OBJECTIVE

To study the effect of Jiangzhuo Mixture (JZM) on glucose and lipid metabolism, free fatty acid (FFA) and insulin sensitivity index of sodium glutamate (MSG) induced obese rats.

METHODS

Sixty-four male MSG rats, 8-10 weeks old, were randomly divided into 4 groups equally, the low dose and high dose JZM group were treated respectively with 10 mL/(kg d) and 20 mL/(kg d) JZM, the rosiglitazone (RGZ) group with water solution of RGE 20 mg/(kg d), while the model group fed only with distilled water 10 mL/(kg d) for control, all by gastrogavage for 7 successive weeks. Levels of fasting blood glucose (FBG), triglyceride (TG), cholesterol (TC), and serum fasting insulin (Ins) were measured before and after treatment. Besides, the level of FFA and the proportion of fat weight to body weight (F/B) were measured and insulin sensitivity index (ISI) was calculated after treatment.

RESULTS

After being treated for 7 weeks, the indexes including TC, FBG, Ins, F/B and FFA were all lower than those in the model group. Compared with before treatment, TC level lowered, FBG and Ins level raised in the two JZM groups and the RGZ group. Comparisons between the three treated group showed a significant lower level of Ins in the RGZ group. Level of ISI was significantly lowered in the 3 groups after treatment (P <0.05), but still higher in the RGZ group than that in the model control group (P <0.05). As for level of FFA, it was 314.81 +/- 110.25 micromol/L in the high dose JZM group and 305.56 +/- 92.33 micromol/L in the RGZ group, which were lower than that in the low dose JZM group (375.00 +/- 219.95 micromol/L, P <0.05).

CONCLUSIONS

JZM could decrease the serum levels of TC, FBG, Ins and FFA in MSG rats, and decrease the fat content of organism in rats' growth process. The FFA decreasing action is dose-dependent. But its effect on ISI in MSG rats in the growth stage is insignificant. JZM can not reverse the forming processes of hyperglycemia, hyperinsulinemia, and insulin resistance in MSG rats.

摘要

目的

研究降浊合剂（JZM）对谷氨酸钠（MSG）诱导的肥胖大鼠糖脂代谢、游离脂肪酸（FFA）及胰岛素敏感指数的影响。

方法

将64只8 - 10周龄雄性MSG大鼠随机均分为4组，低剂量JZM组和高剂量JZM组分别按10 mL/(kg·d)和20 mL/(kg·d)给予JZM灌胃，罗格列酮（RGZ）组按20 mg/(kg·d)给予RGZ水溶液灌胃，模型组仅给予10 mL/(kg·d)蒸馏水灌胃作为对照，连续灌胃7周。治疗前后分别检测空腹血糖（FBG）、甘油三酯（TG）、胆固醇（TC）及血清空腹胰岛素（Ins）水平。此外，治疗后检测FFA水平及脂肪重量占体重的比例（F/B），并计算胰岛素敏感指数（ISI）。

结果

治疗7周后，TC、FBG、Ins、F/B及FFA等指标均低于模型组。与治疗前比较，两个JZM组和RGZ组TC水平降低，FBG和Ins水平升高。三个治疗组比较，RGZ组Ins水平显著降低。治疗后3组ISI水平均显著降低（P <0.05），但RGZ组仍高于模型对照组（P <0.05）。FFA水平方面，高剂量JZM组为314.81±110.25 μmol/L，RGZ组为305.56±92.33 μmol/L，均低于低剂量JZM组（375.00±219.95 μmol/L，P <0.05）。