Ogiwara Kikumi, Hata Keishi
Department of Pathology, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa, 229-8501, Japan.
J Nat Med. 2009 Jul;63(3):323-6. doi: 10.1007/s11418-009-0319-7. Epub 2009 Feb 13.
Electron microscopic observation revealed that lupeol induced melanosome maturation in B16 2F2 mouse melanoma cells and we therefore studied the effects of lupeol on the intracellular events responsible for melanosome transport. Incubation with lupeol for 8 h attenuated the actin stress fiber assembly in B16 2F2 mouse melanoma cells, resulting in dendritic formation in the cells. Longer exposure to lupeol (48 h) increased the expression of tyrosinase, MITF (a specific transcription factor for tyrosinase), Rab27a, and myosin-Va, which are required for melanosome transport.
电子显微镜观察显示，羽扇豆醇可诱导B16 2F2小鼠黑色素瘤细胞中的黑素体成熟，因此我们研究了羽扇豆醇对负责黑素体运输的细胞内事件的影响。用羽扇豆醇孵育8小时可减弱B16 2F2小鼠黑色素瘤细胞中的肌动蛋白应激纤维组装，导致细胞中形成树突状。长时间暴露于羽扇豆醇（48小时）可增加酪氨酸酶、MITF（酪氨酸酶的一种特异性转录因子）、Rab27a和肌球蛋白-Va的表达，这些都是黑素体运输所必需的。
