豚鼠视上核内免疫细胞化学鉴定的血管加压素和催产素神经元的内向整流 (I)。
Inward Rectification (I) in Immunocytochemically-ldentified Vasopressin and Oxytocin Neurons of Guinea-Pig Supraoptic Nucleus.
机构信息
Department of Physiology, The Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, Oregon 97201-3098, USA.
出版信息
J Neuroendocrinol. 1990 Jun 1;2(3):261-5. doi: 10.1111/j.1365-2826.1990.tb00402.x.
Intracellular recordings of magnocellular neurons from the supraoptic nucleus of guinea-pigs were made with KCI/K citrate- and biocytin-filled electrodes. Fifty of 99 cells exhibited a time-dependent inward rectification (TDR). The TDR was activated during hyperpolarizing current pulses to membrane potentials more hyperpolarized than -75 mV. In voltage-clamp recordings, an inward current appeared at voltage steps more hyperpolarized than -75 mV, with properties similar to the slow inward rectifier (I(h)) described in other tissues. The I(h) was blocked by 2 mM CsCI. BaCI(2) (100 to 500 muM) did not block the I(h). Immunocytochemical identification of the recorded cells revealed that both vasopressin (AVP)- and oxytocin (OT)- containing neurons exhibited an I(h).
用 KCI/K 柠檬酸盐和生物胞素填充的电极记录豚鼠视上核的大细胞神经元的细胞内记录。99 个细胞中有 50 个表现出时间依赖性内向整流（TDR）。TDR 在超极化电流脉冲期间被激活，超极化程度超过-75 mV。在电压钳记录中，在超极化程度超过-75 mV 的电压阶跃处出现内向电流，其特性与在其他组织中描述的慢内向整流器（I（h））相似。2 mM CsCI 可阻断 I（h）。BaCI（2）（100 至 500 μM）不能阻断 I（h）。记录细胞的免疫细胞化学鉴定表明，同时含有加压素（AVP）和催产素（OT）的神经元均表现出 I（h）。
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