University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin Hospital, Heidelberg, Victoria, Australia.
J Neuroendocrinol. 1991 Feb 1;3(1):11-4. doi: 10.1111/j.1365-2826.1991.tb00232.x.
The distribution of messenger ribonucleic acid (mRNA) encoding preproneuropeptide Y (prepro-NPY) in the hypothalamus of rats subjected to food deprivation or dehydration has been investigated by quantitative in situ hybridization. Levels of prepro-NPY mRNA in the arcuate nucleus (ARC) were selectively increased by both treatments. The very high concentration of prepro-NPY mRNA seen following 96 h of food deprivation had returned towards control levels after 24 h of refeeding. Levels of preprogalanin (prepro-GAL) mRNA throughout the hypothalamus were essentially unaffected by both regimes. These results demonstrate that hypothalamic NPY gene expression is regulated by peripheral metabolic status (and osmolality), and confirm the key physiological role of NPY in controlling ingestive behaviour.
通过定量原位杂交研究了禁食或脱水的大鼠下丘脑中介导前神经肽 Y（prepro-NPY）的信使核糖核酸（mRNA）的分布。两种处理均选择性地增加了弓状核（ARC）中的 prepro-NPY mRNA 水平。禁食 96 小时后观察到的 prepro-NPY mRNA 浓度非常高，在重新喂食 24 小时后已恢复至对照水平。两种方案对整个下丘脑的 preprogalanin（prepro-GAL）mRNA 水平基本没有影响。这些结果表明，下丘脑 NPY 基因表达受外周代谢状态（和渗透压）调节，并证实 NPY 在控制摄食行为中的关键生理作用。
