Department of Physiology, School of Medicine, Oregon Health Sciences University, Portland, Oregon 97201, USA.
J Neuroendocrinol. 1991 Dec 1;3(6):679-84. doi: 10.1111/j.1365-2826.1991.tb00333.x.
Abstract Using a microdissection technique we localized androgen receptors and aromatase activity (AA) in the brain of male guinea-pigs. In addition, we evaluated the effects of castration and androgen replacement on androgen receptor dynamics and induction of AA. In the castrate animal, cytosolic androgen receptor content was highest in the basal hypothalamus, specifically in the median eminence-arcuate nucleus (> 15 fmol mg protein (1)), while lesser levels were found in the preoptic regions and amygdala. Nuclear receptor content was highest (> 150 fmol mg DNA (-1)) in the median eminence-arcuate nucleus, periventricular region of the preoptic area and cortical amygdala. All regions investigated showed a significant decrease in nuclear receptors following castration and an increase with androgen replacement. However, reciprocal changes in cytosolic androgen receptors were not always evident. Aromatase activity was high in the cortical amygdala, medial amygdala, periventricular region of the preoptic area and bed nucleus of the stria terminalis. Castration and androgen replacement had significant stimulatory effects on AA in the ventral medial hypothalamus, median eminence-arcuate nucleus, cortical amygdala and periventricular regions of the preoptic area and anterior hypothalamus. Thus, androgen receptors and AA are unevenly distributed throughout the subcortical regions of the male guinea-pig brain and respond differently to endocrine stimuli. Our data demonstrate that AA is androgen-dependent in some subcortical regions which contain androgen receptors. Even though nuclear receptors in all brain regions were affected by castration and dihydrotestosterone treatment, the events were not always linked to AA regulation. Due to this difference in regulation, AA may serve divergent functions in guinea-pig brain.
摘要 我们采用显微解剖技术，对雄性豚鼠脑内的雄激素受体（androgen receptor）和芳香化酶活性（aromatase activity，AA）进行了定位。此外，我们还评估了去势和雄激素替代对雄激素受体动力学以及 AA 诱导的影响。在去势动物中，胞质雄激素受体含量在基底下丘脑最高，特别是在正中隆起-弓状核（> 15 fmol mg 蛋白（1）），而在视前区和杏仁核中含量较低。核受体含量在正中隆起-弓状核、视前区脑室周围区和皮质杏仁核中最高（> 150 fmol mg DNA（-1））。所有研究区域的核受体在去势后均显著下降，雄激素替代后增加。然而，胞质雄激素受体的反向变化并不总是明显。AA 活性在皮质杏仁核、内侧杏仁核、视前区脑室周围区和终纹床核中较高。去势和雄激素替代对腹内侧下丘脑、正中隆起-弓状核、皮质杏仁核和视前区脑室周围区以及下丘脑前部的 AA 有显著的刺激作用。因此，雄激素受体和 AA 在雄性豚鼠脑的皮质下区域分布不均，对内分泌刺激的反应不同。我们的数据表明，AA 在一些含有雄激素受体的皮质下区域依赖于雄激素。尽管所有脑区的核受体都受到去势和二氢睾酮处理的影响，但这些事件并不总是与 AA 调节相关。由于这种调节上的差异，AA 可能在豚鼠脑中发挥不同的功能。
