Mullen Thomas E, Kaygun Handan, Marzluff William F
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
Methods Enzymol. 2008;449:23-45. doi: 10.1016/S0076-6879(08)02402-6.
Replication-dependent histone mRNAs are coordinately regulated in parallel with DNA replication. Histone mRNAs accumulate to high levels only in S-phase cells and are degraded rapidly at the end of S phase or when DNA replication is inhibited in S-phase cells. The unique 3' end on histone mRNAs is the cis element responsible for the regulation of histone mRNA degradation. This chapter describes the approaches used to demonstrate the connection between translation of histone mRNA and its degradation as well as the pathway of histone mRNA degradation in mammalian cells. In particular, the initial step in histone mRNA degradation is attachment of an oligo(U) tail to the 3' end of histone mRNA, providing a platform for binding factors that trigger mRNA degradation.
依赖复制的组蛋白mRNA与DNA复制同步受到协调调控。组蛋白mRNA仅在S期细胞中积累至高水平，并在S期末尾或S期细胞中DNA复制受到抑制时迅速降解。组蛋白mRNA独特的3'末端是负责调控组蛋白mRNA降解的顺式元件。本章描述了用于证明组蛋白mRNA翻译与其降解之间联系的方法，以及哺乳动物细胞中组蛋白mRNA的降解途径。特别是，组蛋白mRNA降解的起始步骤是在组蛋白mRNA的3'末端连接一个寡聚（U）尾，为触发mRNA降解的结合因子提供一个平台。
