基于 RAPD 的地中海牛至质量控制方法及其对生药学技术的贡献。

RAPD-based method for the quality control of Mediterranean oregano and its contribution to pharmacognostic techniques.

机构信息

Department of Evolutionary and Functional Biology, University of Parma, Viale G. P. Usberti 11/A, I-43100 Parma, Italy.

出版信息

J Agric Food Chem. 2009 Mar 11;57(5):1835-40. doi: 10.1021/jf8032649.

PMID:19216531

Abstract

A pharmacognostic survey of 84 commercial samples of Mediterranean oregano, obtained from wholesale traders between 2001 and 2007, pinpointed the presence of extraneous plant material in 90.5% of the samples. In 59% of them extraneous material of plant origin was above 20%. Two major groups of botanical foreign matter were identified: oregano-like flavored plants ( Satureja montana L., Origanum majorana L.) and plants lacking a clearly detectable essential oil profile ( Rubus sp., Cistus incanus L., Rhus coriaria L.), added as bulk extraneous material. A random amplified polymorphic DNA (RAPD) method was developed to make the detection of the second group of adulterants easier and speed pharmacognostic analysis of large batches of samples. Thirteen primers discriminating between Origanum spp. and Rubus caesius , R.coriaria, and C. incanus were individuated, allowing their detection in oregano samples with a limit of detection of 1%. The utilization of RAPD as a reliable test to probe the authenticity of Mediterranean oregano or previously screen the presence of specific contaminants is proposed as a complementary approach to pharmacognostic and phytochemical screening.

摘要

对 2001 年至 2007 年间从批发商处获得的 84 个商业样本的地中海牛至进行了生药学调查，发现 90.5%的样本中存在外来植物材料。在其中 59%的样本中，外来植物材料的含量超过 20%。鉴定出了两类主要的植物外来杂质：具有牛至风味的植物（山艾属植物 Satureja montana L.、马郁兰 Origanum majorana L.）和没有明显可检测精油特征的植物（悬钩子属植物 Rubus sp.、滨藜 Cistus incanus L.、漆树 Rhus coriaria L.），它们被添加为散装外来杂质。开发了一种随机扩增多态性 DNA (RAPD) 方法，以更轻松地检测第二组掺杂物，并加快对大量样本的生药学分析。鉴定出了 13 个可区分牛至属和欧洲黑莓 Rubus caesius 、漆树 Rhus coriaria 和滨藜 C. incanus 的引物，可将其检测到牛至样品中的检测限为 1%。建议将 RAPD 用作探测地中海牛至真实性或先前筛选特定污染物存在的可靠测试，作为生药学和植物化学筛选的补充方法。