Dipartimento di Bioscienze, Università degli Studi di Parma, Parco delle Scienze 11/A, I-43124 Parma, Italy.
J Agric Food Chem. 2012 Nov 7;60(44):10998-1004. doi: 10.1021/jf303106r. Epub 2012 Oct 23.
A method based on sequence-characterized amplified regions (SCARs) was developed from random amplified polymorphic DNA markers (RAPDs) specific for Arnica montana L., Bixa orellana L., Calendula officinalis L., Carthamus tinctorius L., Crocus vernus L. (Hill), Curcuma longa L., and Hemerocallis sp. to detect these common bulking agents in commercial saffron (Crocus sativus). The method enabled the unequivocal detection of low amounts (up to 1%) of each adulterant, allowing the preemptive rejection of suspect samples. Its enforcement limits the number of samples to be subjected to further evaluation with pharmacognostic or phytochemical analyses, especially when multiple batches have to be evaluated in a short time. The dimension of the amplicons is suitable for the analysis of degraded DNA obtained from dried, stored, processed, and finely ground commercial material. Proper SCAR markers may represent a fast, sensitive, reliable, and low-cost screening method for the authentication of dried commercial saffron material.
开发了一种基于序列特征扩增区域（SCAR）的方法，该方法针对毛蕊花、胭脂树、金盏花、红花、番红花（Hill）、姜黄和萱草属植物等常见的商业藏红花增稠剂，从随机扩增多态性 DNA 标记（RAPD）特异性标记中获得。该方法能够明确检测到低含量（高达 1%）的每种掺杂物，从而可以预先拒绝可疑样品。其实施限制了需要进行更多药代动力学或植物化学分析的样品数量，尤其是在短时间内必须评估多个批次的情况下。扩增子的大小适合分析从干燥、储存、加工和精细研磨的商业材料中获得的降解 DNA。合适的 SCAR 标记物可能代表一种快速、敏感、可靠且低成本的筛选方法，用于鉴定干燥的商业藏红花材料。
