经工程改造以释放腺苷的人骨髓间充质干细胞移植可减少CA3选择性癫痫发生小鼠模型中的慢性癫痫发作。
Human mesenchymal stem cell grafts engineered to release adenosine reduce chronic seizures in a mouse model of CA3-selective epileptogenesis.
作者信息
Li Tianfu, Ren Gaoying, Kaplan David L, Boison Detlev
机构信息
Robert Stone Dow Neurobiology Laboratories, Legacy Research, 1225 NE 2nd Avenue, Portland, OR 97232, USA.
出版信息
Epilepsy Res. 2009 Apr;84(2-3):238-41. doi: 10.1016/j.eplepsyres.2009.01.002. Epub 2009 Feb 12.
Abstract
A novel generation of silk-based brain implants engineered to release adenosine was recently shown to provide robust seizure suppression in kindled rats. As a first step to develop stem cell-coated silk-based 3D-scaffolds for the therapeutic long-term delivery of adenosine we engineered human mesenchymal stem cells (hMSCs) to release adenosine. Here we demonstrate reduction of chronic seizures in a mouse model of CA3-selective epileptogenesis after infrahippocampal transplantation of adenosine-releasing hMSCs.
摘要
最近，新一代经设计可释放腺苷的丝基脑植入物已被证明能在点燃大鼠中有效抑制癫痫发作。作为开发用于腺苷长期治疗性递送的干细胞包被丝基3D支架的第一步，我们对人间充质干细胞（hMSCs）进行了工程改造，使其能够释放腺苷。在此，我们展示了在海马下移植释放腺苷的hMSCs后，CA3选择性癫痫发生小鼠模型中的慢性癫痫发作减少。
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