Wang Qiaochun, Valkonen Jari P T
College of Horticulture, Northwest Agricultural & Forest University, Yangling 712100, Shaanxi, People's Republic of China.
Trends Plant Sci. 2009 Mar;14(3):119-22. doi: 10.1016/j.tplants.2008.11.010. Epub 2009 Feb 11.
Cryotherapy is a novel application of plant cryopreservation techniques that allows pathogen eradication at a high frequency. It eliminates plant pathogens such as viruses, phytoplasmas and bacteria by briefly treating shoot tips in liquid nitrogen using cryopreservation protocols. Healthy plants are regenerated from the surviving pathogen-free meristematic tissue. The method facilitates treatment of large numbers of samples and is independent of shoot tip size. It has the potential to replace more traditional methods like meristem culture.
冷冻疗法是植物冷冻保存技术的一种新应用，它能够高频率地根除病原体。通过使用冷冻保存方案在液氮中对茎尖进行短暂处理，可消除植物病原体，如病毒、植原体和细菌。从存活的无病原体分生组织中再生出健康的植株。该方法便于处理大量样本，且与茎尖大小无关。它有潜力取代诸如分生组织培养等更传统的方法。
