Sakurai J, Tsuchiya Y, Ochi S, Fujii Y
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University.
Microbiol Immunol. 1991;35(6):481-6. doi: 10.1111/j.1348-0421.1991.tb01578.x.
The effect of Clostridium perfringens alpha toxin on contraction induced by electric stimulation of isolated guinea-pig diaphragm was investigated. The toxin inhibited electrically stimulated contraction of the tissue in a dose- and incubation time-dependent manner. Tetrodotoxin resulted in no effect of the action of the toxin. Nifedipine dose-dependently delayed the action of the toxin, but verapamil and diltiazem did not. On the other hand, treatment of the toxin with N-acetylimidazole caused significant reduction of the inhibitory activity of the toxin on contraction, but did not cause significant loss of phospholipase C activity (PN activity) as measured by hydrolysis of p-nitrophenylphosphorylcholine. The data showed that the toxin impairs contraction of isolated guinea-pig diaphragm.
研究了产气荚膜梭菌α毒素对豚鼠离体膈肌电刺激诱导收缩的影响。该毒素以剂量和孵育时间依赖性方式抑制组织的电刺激收缩。河豚毒素对毒素的作用无影响。硝苯地平剂量依赖性地延迟毒素的作用，但维拉帕米和地尔硫䓬则无此作用。另一方面，用N-乙酰咪唑处理毒素会导致其对收缩的抑制活性显著降低，但通过对硝基苯磷酸胆碱水解测定的磷脂酶C活性（PN活性）并未显著丧失。数据表明该毒素损害豚鼠离体膈肌的收缩。
