Kaur Baljit, Jørgensen Aud, Duttaroy Asim K
Department of Nutrition, Faculty of Medicine, Institute for Basic Medical Sciences, University of Oslo, PO Box 1046 Blindern, N-0316 Oslo, Norway.
Prostaglandins Leukot Essent Fatty Acids. 2009 Feb-Mar;80(2-3):93-9. doi: 10.1016/j.plefa.2009.01.002. Epub 2009 Feb 14.
In order to exert metabolic effects, fatty acids must be taken up by cells and metabolize effectively to different classes of cellular lipids (triacylglycerols, phospholipids, etc.) for incorporation into different cellular and intracellular compartments. Therefore, the main aim of the present study is to investigate the uptake and metabolism of fatty acids representing three different series of fatty acids such as oleic acid, 18:1n-9 (OA), arachidonic acid, 20:4n-6 (AA), and eicosapentaneoic acid, 20:5n-3 (EPA) by breast cancer cells, MDA-MB-231. Moreover, we investigated the effects of insulin and several adipokines on the fatty acid uptake by these cells as obesity and insulin resistance syndrome have been suggested to affect breast cancer risk. We report for the first time that AA was predominantly taken up by these cells compared with EPA and OA. Pre-incubation of these cells with TNFalpha stimulated most of the uptake of EPA (30%), whereas uptake of OA and AA was stimulated only 10-15% compared with the controls. Insulin, leptin, and adiponectin had no effect on fatty acid uptake by these cells. Together these results demonstrate that preferential uptake of AA in MDA-MB-231 cells, and the fatty acid uptake activity of these cells is influenced by TNFalpha.
为了发挥代谢作用，脂肪酸必须被细胞摄取并有效代谢为不同种类的细胞脂质（三酰甘油、磷脂等），以便纳入不同的细胞和细胞内区室。因此，本研究的主要目的是研究乳腺癌细胞MDA-MB-231对代表三种不同系列脂肪酸的摄取和代谢情况，这三种脂肪酸分别是油酸、18:1n-9（OA）、花生四烯酸、20:4n-6（AA）和二十碳五烯酸、20:5n-3（EPA）。此外，由于肥胖和胰岛素抵抗综合征被认为会影响乳腺癌风险，我们研究了胰岛素和几种脂肪因子对这些细胞脂肪酸摄取的影响。我们首次报告，与EPA和OA相比，这些细胞主要摄取AA。用肿瘤坏死因子α（TNFα）预孵育这些细胞，刺激了大部分EPA的摄取（30%），而与对照组相比，OA和AA的摄取仅刺激了10 - 15%。胰岛素、瘦素和脂联素对这些细胞的脂肪酸摄取没有影响。这些结果共同表明，MDA-MB-231细胞对AA有优先摄取，且这些细胞的脂肪酸摄取活性受TNFα影响。
