Lever Michael, Atkinson Wendy, Slow Sandy, Chambers Stephen T, George Peter M
Biochemistry Unit, Canterbury Health Laboratories, Christchurch, New Zealand.
Clin Biochem. 2009 May;42(7-8):706-12. doi: 10.1016/j.clinbiochem.2009.02.001. Epub 2009 Feb 13.
We aimed to compare the individuality (within subject consistency) of plasma and urine betaine and N,N-dimethylglycine.
In two separate groups of 8 males (ages 19 to 40), plasma (10) and urine (6) samples were collected either over a single day or over an 8 week period. The individuality of the betaine and N,N-dimethylglycine plasma concentrations and excretions were estimated by one-way repeated measures analysis of variance. The reliability coefficients and indices of individuality were calculated. The between-subject variation in the study population was compared with that in a normal population (n=192 for plasma, 205 for urine).
Plasma betaine concentrations were significantly different between subjects over 24 h and 8 weeks (p<0.00001). Plasma dimethylglycine concentrations were different over 24 h. Urine betaine and dimethylglycine excretions were different in both (p<0.0001). Betaine was more individual than dimethylglycine in both plasma and urine. Compared with a normal healthy population, the between-subject variation in plasma betaine was less (p<0.001) in the study group, but similar for dimethylglycine and for urine betaine.
Plasma betaine and urinary betaine excretions are more individual than dimethylglycine. Plasma and urine betaine are highly individual in the general population.
我们旨在比较血浆和尿液中甜菜碱及N，N - 二甲基甘氨酸的个体差异（受试者内一致性）。
在两组各8名男性（年龄19至40岁）中，分别在一天内或8周期间采集血浆（10份）和尿液（6份）样本。通过单因素重复测量方差分析评估甜菜碱和N，N - 二甲基甘氨酸血浆浓度及排泄量的个体差异。计算可靠性系数和个体差异指数。将研究人群的受试者间变异与正常人群（血浆192例，尿液205例）进行比较。
24小时及8周内，受试者间血浆甜菜碱浓度存在显著差异（p<0.00001）。24小时内血浆二甲基甘氨酸浓度存在差异。尿液中甜菜碱和二甲基甘氨酸排泄量在两者中均存在差异（p<0.0001）。血浆和尿液中，甜菜碱的个体差异均大于二甲基甘氨酸。与正常健康人群相比，研究组血浆甜菜碱的受试者间变异较小（p<0.001），但二甲基甘氨酸及尿液甜菜碱与之相似。
血浆甜菜碱和尿液甜菜碱排泄量的个体差异大于二甲基甘氨酸。在一般人群中，血浆和尿液甜菜碱具有高度个体差异。
