Han Fang-xuan, Lin Hui, Ru Ling
Department of Pharmacy, People's Hospital of Hainan Province, Haikou 570311, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Jan;29(1):97-9.
To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu).
The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay.
5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities.
MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.
评估MTT比色法检测乳腺癌细胞对5-氟尿嘧啶（5-Fu）体外化疗敏感性的可行性。
采用MTT法评估人乳腺癌细胞系MCF-7和MDA-MB-435S对不同浓度5-Fu的化疗敏感性。
5-Fu处理导致具有低转移能力和高转移能力的乳腺癌细胞生长受到剂量依赖性抑制。
MTT法可能有助于选择合适的化疗药物，并为乳腺癌个体化化疗提供依据。
