Koenig R, Loss S, Specht J, Varrelmann M, Lüddecke P, Deml G
Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
Institut für Zuckerrübenforschung, Abteilung Phytomedizin, Holtenser Landstraße 77, D-37079 Göttingen, Germany.
J Gen Virol. 2009 Mar;90(Pt 3):759-763. doi: 10.1099/vir.0.007112-0.
Beet necrotic yellow vein virus (BNYVV) A type isolates E12 and S8, originating from areas where resistance-breaking had or had not been observed, respectively, served as starting material for studying the influence of sequence variations in BNYVV RNA 3 on virus accumulation in partially resistant sugar beet varieties. Sub-isolates containing only RNAs 1 and 2 were obtained by serial local lesion passages; biologically active cDNA clones were prepared for RNAs 3 which differed in their coding sequences for P25 aa 67, 68 and 129. Sugar beet seedlings were mechanically inoculated with RNA 1+2/RNA 3 pseudorecombinants. The origin of RNAs 1+2 had little influence on virus accumulation in rootlets. E12 RNA 3 coding for V(67)C(68)Y(129) P25, however, enabled a much higher virus accumulation than S8 RNA 3 coding for A(67)H(68)H(129) P25. Mutants revealed that this was due only to the V(67) 'GUU' codon as opposed to the A(67) 'GCU' codon.
甜菜坏死黄脉病毒（BNYVV）A型分离株E12和S8分别源自抗性突破已被观察到或未被观察到的地区，作为研究BNYVV RNA 3序列变异对部分抗性甜菜品种中病毒积累影响的起始材料。通过连续局部病斑传代获得仅含有RNA 1和2的亚分离株；针对P25氨基酸67、68和129编码序列不同的RNA 3制备了具有生物活性的cDNA克隆。用RNA 1+2/RNA 3假重组体对甜菜幼苗进行机械接种。RNA 1+2的来源对根中病毒积累影响很小。然而，编码V(67)C(68)Y(129) P25的E12 RNA 3比编码A(67)H(68)H(129) P25的S8 RNA 3能使病毒积累量高得多。突变体表明，这仅归因于V(67)的“GUU”密码子，而不是A(67)的“GCU”密码子。
