Al-Saleh Esmaeil S, Obuekwe Christian
Microbiology Program, Department of Biological Sciences, Safat, College of Science, Kuwait University, State of Kuwait.
J Basic Microbiol. 2009 Jun;49(3):256-63. doi: 10.1002/jobm.200800130.
Assessment of nickel contents in soil samples in Kuwait indicated only a minor difference in concentration in hydrocarbon-contaminated (86 mg kg(-1) soil) and non-contaminated soils (84 mg kg(-1) soil). The potential inhibitory effects of nickel on the number of hydrocarbon degraders, and hydrocarbon utilization were investigated over a wide range of nickel concentrations to span concentrations observed in the soil. Nickel addition, as nickel sulphate, to soil samples reduced the number of hydrocarbon degraders in all samples by a wide range (15-96%) depending on concentration and the hydrocarbon substrate utilized. Similarly, the metabolic activities were affected as observed in mineralization (3-60%) of soils amended with various concentrations of nickel. The inhibitive effects of nickel on hexadecane and crude oil utilization were minimal but were significantly higher with naphthalene (P < 0.05) in both hydrocarbon-contaminated and non-contaminated soils. Polarographic determination of hydrocarbons induced oxygen uptake rate demonstrated the tendency of nickel to significantly inhibit (P < 0.05) the oxidation of polyaromatic hydrocarbons compared to aliphatic hydrocarbons.
科威特土壤样本中镍含量的评估表明，受碳氢化合物污染的土壤（86毫克/千克土壤）和未受污染的土壤（84毫克/千克土壤）中的镍浓度仅有微小差异。在很宽的镍浓度范围内研究了镍对碳氢化合物降解菌数量和碳氢化合物利用的潜在抑制作用，该浓度范围涵盖了在土壤中观察到的浓度。向土壤样本中添加硫酸镍形式的镍，根据浓度和所利用的碳氢化合物底物不同，所有样本中碳氢化合物降解菌的数量均大幅减少（15% - 96%）。同样，在添加了不同浓度镍的土壤矿化过程中（3% - 60%）观察到代谢活性受到影响。在受碳氢化合物污染和未受污染的土壤中，镍对十六烷和原油利用的抑制作用最小，但对萘的抑制作用显著更高（P < 0.05）。极谱法测定碳氢化合物诱导的氧气摄取率表明，与脂肪族碳氢化合物相比，镍有显著抑制（P < 0.05）多环芳烃氧化的趋势。
