Detection of apoptosis based on the interaction between annexin V and phosphatidylserine.

The detection of apoptosis based on the interaction between annexin V and phosphatidylserine is presented in this paper. The in vitro experimental results demonstrate that annexin V in polyethylenimine film can maintain its high affinity with phosphatidylserine translocated from the inner to the outer plasma membrane of the apoptotic cells, which may consequently inhibit the redox reaction of Ru(NH(3))(5)Cl at the annexin V and polyethylenimine comodified pyrolytic graphite electrode. Therefore, if Ca(2+), the required ion for the function of annexin V, is added into the test solution, the redox wave of Ru(NH(3))(5)Cl can be clearly changed to indicate apoptosis and a simple and convenient method to detect apoptosis is developed.