Todd J D, Curson A R J, Dupont C L, Nicholson P, Johnston A W B
School of Biological Sciences, University of East Anglia, Norwich, UK.
Environ Microbiol. 2009 Jun;11(6):1376-85. doi: 10.1111/j.1462-2920.2009.01864.x. Epub 2009 Feb 12.
The marine alphaproteobacterium Roseovarius nubinhibens ISM can produce the gas dimethyl sulfide (DMS) from dimethylsulfoniopropionate (DMSP), a widespread secondary metabolite that occurs in many phytoplankton. Roseovarius possesses a novel gene, termed dddP, which when cloned, confers on Escherichia coli the ability to produce DMS. The DddP polypeptide is in the large family of M24 metallopeptidases and is wholly different from two other enzymes, DddD and DddL, which were previously shown to generate DMS from dimethylsulfoniopropionate. Close homologues of DddP occur in other alphaproteobacteria and more surprisingly, in some Ascomycete fungi. These were the biotechnologically important Aspergillus oryzae and the plant pathogen, Fusarium graminearum. The dddP gene is abundant in the bacterial metagenomic sequences in the Global Ocean Sampling Expedition. Thus, dddP has several novel features and is widely dispersed, both taxonomically and geographically.
海洋α-变形菌新鞘氨醇菌ISM能够从二甲基巯基丙酸内盐(DMSP)产生气体二甲基硫醚(DMS),DMSP是一种在许多浮游植物中广泛存在的次生代谢产物。新鞘氨醇菌拥有一个名为dddP的新基因,该基因克隆后可使大肠杆菌具备产生DMS的能力。DddP多肽属于M24金属肽酶大家族,与另外两种先前已证明可从二甲基巯基丙酸内盐生成DMS的酶DddD和DddL完全不同。DddP的紧密同源物存在于其他α-变形菌中,更令人惊讶的是,还存在于一些子囊菌真菌中。这些真菌包括具有重要生物技术意义的米曲霉和植物病原菌禾谷镰刀菌。dddP基因在全球海洋采样探险的细菌宏基因组序列中大量存在。因此,dddP具有几个新特性,并且在分类学和地理分布上都广泛存在。